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Enterovirus type-71 nucleic acid amplification fluorescent quantitative and liquid chip dual test kit

An enterovirus, liquid chip technology, applied in fluorescence/phosphorescence, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problem of insufficient detection methods for enterovirus type 71, and achieve excellent specificity and sensitivity. , Easy to operate, stable results

Inactive Publication Date: 2010-03-10
SHANGHAI GENEPROTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is that the existing enterovirus 71 detection methods all have relatively large deficiencies

Method used

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  • Enterovirus type-71 nucleic acid amplification fluorescent quantitative and liquid chip dual test kit
  • Enterovirus type-71 nucleic acid amplification fluorescent quantitative and liquid chip dual test kit

Examples

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Embodiment Construction

[0038] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. For the experimental methods without specific conditions indicated in the following examples, the conventional conditions or the conditions suggested by the manufacturer are usually followed. Ratios and percentages are by weight unless otherwise indicated.

[0039] The present invention is further illustrated below with examples, but the present invention is not limited thereto.

[0040] (1) Detection of clinical samples by fluorescence quantitative PCR EV71 detection reagent (liquid chip detection is performed simultaneously, and will be described separately here)

[0041](1) Design of primers and probes: A pair of primers and a Taq-Man probe were designed for the EV71 genome-specific conserved sequence, and the probe was ...

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Abstract

The invention belongs to the field of biotechnology and particularly relates to an enterovirus type-71 test kit. The invention discloses an enterovirus type-71 nucleic acid amplification fluorescent quantitative and liquid chip dual test kit, which comprises: a pair of EV type-71 specific primers A and A' having biotin-marked 5'-terminals; a pair of EV type-71 specific primers B and B' having biotin-marked 5'-terminals; fluorescence-encoded microspheres C cross-linked with probes F matched with the sequence of amplified fragments of the primers A and A'; fluorescence-encoded microspheres D cross-linked with probes G matched with the sequence of amplified fragments of the primers B and B'; a pair of EV type-71 specific conserved sequence primers E and E'; a Taq-Man probe having a fluorescent group-marked 5'-terminal and a BHQ-marked 3'-terminal; and QRT-PCR reagent and fluorescence-marked avidin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a detection kit for enterovirus 71. Background technique [0002] Enterovirus 71 (EV71) is a member of the genus Enterovirus in the family Picornaviridae. EV71 infection mainly causes hand-foot-mouth disease (HFMD) in patients and is the main pathogen of the disease. In addition, EV71 can also cause aseptic meningitis, brainstem encephalitis, and polio-like paralysis and other nervous system-related diseases, and can cause outbreaks, leading to death, and seriously endangering human health. [0003] In order to quickly identify the cause of an EV71-related disease outbreak and take correct and effective control measures in a timely manner, new diagnostic methods that are accurate, rapid, highly sensitive, highly specific, and high-throughput for EV71 are urgently needed. Enterovirus infection has the characteristics of "one cause and multiple diseases, one disease and m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68G01N21/64
Inventor 朱文斯
Owner SHANGHAI GENEPROTECH
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