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Antigen-antibody reaction-based controllable gold nanorod assembling method

A gold nanorod and antibody reaction technology, applied in nanotechnology, nanotechnology, nanostructure manufacturing, etc., to achieve the effect of simple operation methods

Inactive Publication Date: 2012-11-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The experiment is illustrated with gentamicin antigen and antibody

Method used

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  • Antigen-antibody reaction-based controllable gold nanorod assembling method
  • Antigen-antibody reaction-based controllable gold nanorod assembling method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 The side coupling of gentamicin antibody and coating agent to gold nanorods:

[0042] a. Coupling of antibody and gold nanorods: 0.5mL, 100μg / mL gentamicin antibody was added dropwise to 0.5mL, 2nmol / L gold nanorods pre-adjusted to pH 8.5-8.8 with 0.1mol / L potassium carbonate In the rod solution, shake and mix at room temperature, react at 37°C for 1h, centrifuge at 6000rpm for 20min after the reaction, remove unbound antibody, resuspend in 0.01mol / L, pH 7.4 PBS (containing 0.5% PEG2000), and obtain Qingda Gold nanorod probes modified by mycin antibody;

[0043] b. Coupling agent and gold nanorod coupling: the method is similar to that of antibody and gold nanorod coupling, 0.5mL, 100μg / mL gentamicin coating agent is added dropwise and pre-adjusted with 0.1mol / L hydrochloric acid to pH 5, 0.5mL, 2n mol / L gold nanorod solution, shake and mix at room temperature, react at 37°C for 1h, centrifuge at 6000rpm for 20min at the end of the reaction, remove unbound co...

Embodiment 2

[0044] Example 2 Assembly of gold nanorods side by side under the action of antigen and antibody:

[0045]Add 10 μL modified gentamicin antibody-modified gold nanorod solution to 10 μL modified gentamicin-coated original gold nanorod solution, vortex and mix well, incubate at 37°C for 0.5 h, and the reaction is complete. Take 10 μL reaction solution and dilute 5 times The product was characterized by transmission electron microscopy.

Embodiment 3

[0046] Example 3 Coupling Modification of Antibodies and Gold Nanorods:

[0047] A. Modification of Antibody

[0048] The modification of the antibody uses the carbodiimide method to combine lipoic acid and the antibody. The specific steps are as follows:

[0049] ① Mix 0.3mL of lipoic acid ethanol solution (containing 1.625mg of lipoic acid) with 0.4mL of antibody.

[0050] ② Add 75 μL of carbodiimide dropwise into the above solution under the condition of magnetic stirring, and react at room temperature for 4 hours.

[0051] ③ Dialyze with pH 7.4 phosphate buffered saline for 3 days, and change the dialysate 3 times a day.

[0052] ④ Store the modified antibody at 4°C for later use.

[0053] B. Antibody and gold nanorod end-face coupling

[0054] The synthesis of gold nanorods by the seed crystal growth method first needs to be centrifuged at 7500rpm for 15min to remove excess Vc and AgNO in the solution. 3 Gold nanorods with small spherical particles were resuspended i...

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Abstract

The invention discloses an antigen-antibody reaction-based controllable gold nanorod assembling method, which belongs to the technical field of nano immunity. In the invention, the antigen-antibody is directionally coupled to the end face or the side face of the gold nanorods by different methods; and the assembling of the gold nanorods is realized by the identification reaction between the antigen and the antibody. The assembling process is reacted in a liquid environment; the operating method is simple; the controllable composting of the gold nanorod can be realized in a mild condition quickly, so large-scale assembled products can be generated within a certain time.

Description

technical field [0001] The invention relates to a controllable method for assembling gold nanorods based on antigen-antibody reaction, and belongs to the technical field of nanometer immunity. Background technique [0002] Due to the many properties derived from the rich surface plasmon resonance of gold nanorods, today's dispersed gold nanorods are widely used in many fields. Examples include optical data storage, nanosensors, bioimaging, controlled gene delivery, and photothermal therapy for tumors, etc. [0003] Antigen-antibody immune response is one of the most common biological recognition responses, and is often used in immune responses. Today, the development of science and technology requires the use of assembled gold nanorods, one-dimensional, two-dimensional, and even three-dimensional functionalized structures. Because the assembled gold nanorods not only have the properties of a single gold nanorod, but also derive many group properties. These optical and ele...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531B82B3/00
Inventor 胥传来朱颖越陈伟徐丽广马伟彭池方
Owner JIANGNAN UNIV
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