Method for calibrating and measuring activity of adenosine deaminase, kit matched and use thereof

A technology of adenosine deaminase and kit, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, color/spectral characteristic measurement, etc., which can solve the problems of inappropriate clinical biochemical tests, and achieve the goal of improving test accuracy Effect

Inactive Publication Date: 2010-05-19
NINGBO RUI BIO TECH
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Problems solved by technology

[0006] However, the adenosine deaminase dehydrogenase method can quantitatively measure the activity of adenosine de

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  • Method for calibrating and measuring activity of adenosine deaminase, kit matched and use thereof
  • Method for calibrating and measuring activity of adenosine deaminase, kit matched and use thereof
  • Method for calibrating and measuring activity of adenosine deaminase, kit matched and use thereof

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[0041] The following are specific embodiments of the present invention to further describe the technical solutions of the present invention, but the present invention is not limited to these embodiments.

[0042] 1. Determination of adenosine deaminase standard and its activity

[0043] (1) Activity of standard adenosine deaminase 0-150 U / L human gene recombinant adenosine deaminase

[0044] (2) Determination of adenosine deaminase standard activity

[0045] Reagent composition:

[0046] Reagent RI: including 0.015mol / L a-ketoglutaric acid, 0.30×10 -3 mol / L reduced coenzyme I disodium salt, 1.50×10 -3 mol / L adenosine diphosphate monosodium salt and 0.10 mol / L phosphate buffer with a pH of 7.20;

[0047] Reagent RII: 200U / ml glutamate dehydrogenase;

[0048] Reagent RIII: Including 1.20×10 -2 mol / L adenosine, 2.00×10 -5 mol / L disodium edetate, in which reagent RII and reagent RIII are both prepared with pH7.20 phosphate buffer.

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Abstract

The invention provides a method for calibrating and measuring activity of adenosine deaminase, a kit matched and a use thereof. The method comprises the following steps: detecting the kit with a method of removing dehydrogenase by adenosine deaminase, and determining the activity of an adenosine deaminase standard product; calibrating and measuring the activity of the adenosine deaminase of a biological sample by taking the activity of the standard product as the reference according to the Trinder method. The invention has the following advantages: the method solves the deficiency of the Trinder method, improves accuracy, reduces errors of apparatuses, and strengthens comparability of the adenosine deaminase data with the normal reference range; moreover, the method can judge acute liver damage and residual diseases, assist diagnoses of chronic liver disease and liver fibrosis and differentiation of jaundice, a diagnosis of tuberculous hydrothorax and ascites, a diagnosis of central nervous system diseases and differential diagnosis, and a pyrogenic diagnosis caused by typhia and the like according to the activity of the adenosine deaminase; and the method is applied to popularization and application in hospitals.

Description

technical field [0001] The invention belongs to the technical field of medical examination and measurement, and in particular relates to a method for calibrating and measuring the activity of adenosine deaminase, a matched kit and its application. Background technique [0002] Quantitative determination of adenosine deaminase activity with Trinder's method in the prior art is comparatively general in clinical chemical examination, but, the shortcoming of Trinder's method is that the adenosine deaminase standard substance that can not use a kind of recognized standard method to measure, As a result, the test accuracy is reduced, the instrument error caused by the application of different biochemical analyzers in different laboratories is also the main reason for the confusion and incomparability of the normal reference range data of adenosine deaminase. [0003] Adenosine deaminase dehydrogenase method uses adenosine deaminase to decompose adenine nucleotide to generate inosi...

Claims

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Application Information

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IPC IPC(8): C12Q1/34G01N21/31
Inventor 张闻
Owner NINGBO RUI BIO TECH
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