Method for Microalgae Photobiological Cultivation Using Luminescent Particles

Abstract

The invention discloses a method for microalgae photobiological cultivation by using luminescent particles. The luminescent particles with different light quality conversion functions are added to the culture solution for the cultivation of microalgae cells. The luminescent particle of the present invention is prepared by mixing a luminescent agent with a transparent resin, and adding the luminescent particle into a photobioreactor for cultivating microalgae, and realizing the suspension and flow of the particle by stirring. When the luminescent particles flow to the light-receiving surface of the reactor, short-wavelength light (such as 200-400nm) will excite the luminescent agent in the particle, and emit light with a wavelength of 500-760nm required for photosynthesis of microalgae, which improves the utilization of light energy Rate. On the other hand, the luminescent particles excited by the light-receiving surface flow with the fluid into the area where the photoreactor is far away from the light-receiving surface and the light intensity is not enough, and continue to emit light due to the effect of afterglow, increasing the light intensity of these light and dark areas. Thus, the photosynthetic space utilization rate in the microalgae photobioreactor is increased.

Description

technical field [0001] The invention relates to a photobiological reaction culture technology, in particular to a method for cultivating microalgae cells on a large scale by suspending functional luminescent particles prepared by a long-lasting rare earth phosphorescent luminescent agent in a photobioreactor. Background technique [0002] The bioreactor culture of microalgae is an important way to avoid bacterial contamination, increase cell culture density and metabolite yield, and facilitate operation and control. However, light conditions have a crucial impact on the growth and metabolism of microalgal cells. The photosynthetic process of photosynthetic cells is mainly carried out by using light with a wavelength of 400-760nm. Short-wavelength high-energy ultraviolet radiation will cause high toxicity to chlorophyll and cause cells to "bleach" to death. Literature (1) Zhang Songpei, Li Jianyu, Chen Juan, Xiao Yang, Sun Yu'e: Study on the spectral characteristics of light...

AI Technical Summary

Problems solved by technology

[0003] In the above literature or patent technology, the functional light conversion film is used to convert the ultraviolet light that is toxic to plant cells into the red light (640-690nm) and blue light (400-500nm) required by plant photosynthesis, so that to a certain extent The efficiency of photosynthesis is improved. The functional light conversion film prepared by Chinese patent CN1982433A only emits red light, and the emission spectrum is relatively simple.

However, in the actual cultivation process of the photobioreactor, the light intensity on the light-receiving surface is generally relatively high. Excessive light intensity will cause the cells to produce photoinhibition.

On the contrary, in the area away from the light-receiving surface of the reactor, due to the attenuation of the light when it propagates in the medium water body and the shading effect of the cells, the light intensity is insufficient, resulting in light limitation, which makes the light distribution in the large-diameter reactor seriously uneven.

Therefore, traditional photobioreactors mostly adopt narrow tube or thin plate type, resulting in high cost of photobioreactors, large floor area, difficult enlargement, and high cost of photosynthetic cell culture.