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Combined test reagent card for cytomegalovirus and rubella virus

A cytomegalovirus and rubella virus technology, applied in the field of combined detection cards, can solve the problems of many influencing factors, prone to false positives, and short duration of IgM antibody detection, achieving high accuracy, broad application prospects, and reducing procedures. Effect

Active Publication Date: 2013-02-27
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the laboratories use the single index of IgM antibody in early pregnancy serum as the index of cytomegalovirus and rubella virus infection in pregnant women, but there are problems only from the perspective of methodology, because there are many factors affecting the detection of IgM antibody, mainly false positives are prone to occur, and Antibody is the earliest to be produced, once infected, it is produced quickly, and it works against infection in the early stage of infection, but it lasts for a short time and disappears quickly

Method used

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  • Combined test reagent card for cytomegalovirus and rubella virus
  • Combined test reagent card for cytomegalovirus and rubella virus
  • Combined test reagent card for cytomegalovirus and rubella virus

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1: as figure 1 with figure 2 As shown, a detection card for combined cytomegalovirus and rubella virus detection reagents includes a card cover 1, a card bottom 2 and a test strip 3, and the card cover 1 is provided with a sampling hole 11, an observation hole 12 and an installation mark hole 13 , The inside of the card base 2 is provided with a groove 21 for placing the test strip 3 . The detection sample addition area 31 on the test strip 3 corresponds to the sample addition hole 11 on the card cover 1; correspond.

[0044] As pictured and Figure 4As shown, the test strip 3 is successively provided with a detection sample area 31, a gold label area 32, a nitrocellulose membrane area 33 and a water absorption area 34, and the detection sample area 31 is a glass fiber layer; 3 The colloidal gold layer 321 on the upper surface, the upper surface of the colloidal gold layer 321 is provided with a protective layer 322; the nitrocellulose membrane area 33 i...

Embodiment 2

[0071] Embodiment 2: A cytomegalovirus and rubella virus combined detection reagent detection card, the structure of which is the same as that of embodiment 1.

[0072] A method for preparing a cytomegalovirus and rubella virus combined detection reagent detection card is assembled from the above-mentioned card cover 1, card bottom 2 and test strip 3, and the test strip 3 is sequentially provided with a detection sample area 31 and a gold label area 32, nitrocellulose membrane area 33 and water absorption area 34, gold label area 32 is provided with colloidal gold layer 321, and colloidal gold layer 321 is made of chloroauric acid solution, trisodium citrate solution, potassium carbonate solution and anti-human IgG monoclonal antibody solution is prepared.

[0073] The preparation process of colloidal gold layer 321 is as follows:

[0074] A. Take 100ml of 4% chloroauric acid aqueous solution, heat to boiling, add 0.8ml of 0.5% trisodium citrate solution, mix quickly, continu...

Embodiment 3

[0085] Embodiment 3: A cytomegalovirus and rubella virus combined detection reagent detection card, the structure of which is the same as that of embodiment 1.

[0086] A method for preparing a cytomegalovirus and rubella virus combined detection reagent detection card is assembled from the above-mentioned card cover 1, card bottom 2 and test strip 3, and the test strip 3 is sequentially provided with a detection sample area 31 and a gold label area 32, nitrocellulose membrane area 33 and water absorption area 34, gold label area 32 is provided with colloidal gold layer 321, and colloidal gold layer 321 is made of chloroauric acid solution, trisodium citrate solution, potassium carbonate solution and anti-human IgG monoclonal antibody solution is prepared.

[0087] The preparation process of colloidal gold layer 321 is as follows:

[0088] A. Take 100ml of 2% chloroauric acid aqueous solution, heat to boiling, add 0.8ml of 0.8% trisodium citrate solution, mix quickly, continu...

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Abstract

The invention discloses a combined test reagent card for cytomegalovirus and rubella virus, which comprises a card cover, a card bottom and a test strip. The test strip is provided with a test sample region, a gold label region, a nitrocellulose membrane region and a water absorption region sequentially; and the nitrocellulose membrane region is provided with a quality-controlling line coated with goat anti mouse IgG, a testing line coated with cytomegalovirus antigen and a testing line coated with rubella virus antigen sequentially. The technical scheme adopted by the invention can test cytomegalovirus IgG antibody and rubella virus IgG antibody in the sample simultaneously and the total accordance rate of the testing result is higher, so the application prospect is wide. Compared with the detection of IgM antibody, the combined test reagent card for the cytomegalovirus and the rubella virus tests the IgG antibody; the IgG antibody can be carried for the whole life and is only associated with acute infection; and the accuracy is high.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a combined detection card capable of simultaneously detecting IgG antibodies to cytomegalovirus (CMV) and IgG antibodies to rubella virus (RV). Background technique [0002] Cytomegalovirus (Cytomegalovirus, CMV), also known as cellular inclusion virus, is named because the infected cells are enlarged and have huge nuclear inclusions. Cytomegalovirus infection is very widespread in the population. The infection rate of adults in my country is over 95%. It is usually a recessive infection. Most infected people have no clinical symptoms, but under certain conditions, it can invade multiple organs and systems and cause serious diseases. The virus can invade the lungs, liver, kidneys, salivary glands, other mammary glands, and multinucleated leukocytes and lymphocytes, and can excrete the virus from saliva, milk, sweat, blood, urine, semen, and uterine secretions for a long time o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/571G01N33/532G01N33/577
Inventor 杨致亭来秀芬刘善利傅爱华范永熙
Owner 山东康华生物医疗科技股份有限公司