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Method for extracting alpha-glucosidase activity inhibitor from plants

A technology for glucosidase and activity inhibitors, which is applied in the field of extraction of α-glucosidase activity inhibitors, can solve the problems of not extracting α-glucosidase activity inhibitors, and achieve the goal of being suitable for large-scale production and reducing diabetes , cheap effect

Inactive Publication Date: 2014-04-02
DALIAN FISHERIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far there is no relevant report about extracting α-glucosidase activity inhibitors from the above three plants

Method used

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  • Method for extracting alpha-glucosidase activity inhibitor from plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] a. Accurately weigh 10 g of dry Tamarix multibranch stems and leaves, chop and grind, then immerse in 100 ml of absolute ethanol solution overnight, use a Soxhlet extractor to heat and reflux for extraction, the extraction temperature is to reach the boiling temperature, and the number of extractions is 2 times, each The extraction time is 2 hours, discard the extract to remove small polar substances such as pigments, and dry the remaining stems and leaves to obtain the extract;

[0018] b. Add 200ml of pure water to the extract and boil for extraction, extract twice, each time for 2h;

[0019] c. Collect the combined and cooled boiled extract, concentrate under reduced pressure at 60° C., and freeze-dry the stem and leaf extract of Tamarix multibranches.

[0020] Get the stem and leaf extract of Tamarix multibranches of Example 1, add distilled water to constant volume, prepare the concentration of Tamarix multibranches extract solution that is 1.5625mg / mL, carry out t...

Embodiment 2

[0022] a. Accurately weigh 5 g of dried Tamarix multibranch stems and leaves, put them into an extractor after being chopped and ground, add 50ml of acetone solution to soak, and use a Soxhlet extractor to extract 3 times, each time for 1 hour, and the extraction temperature is up to Boiling temperature, discard the extract to remove small polar substances such as pigments, and dry the remaining stems and leaves to be the extract;

[0023] b. Add 50ml of pure water to the extract and boil for extraction, extract 3 times, 1 hour each time;

[0024] c. Collect the combined and cooled boiled extract, concentrate under reduced pressure at 60° C., and freeze-dry the stem and leaf extract of Tamarix multibranches.

[0025] Get the stem and leaf extract of Tamarix multibranches in Example 2, add distilled water to constant volume, and prepare a concentration of Tamarix multibranches extract solution that is 0.5 mg / mL, carry out the α-glucosidase inhibitory activity experiment accordi...

Embodiment 3

[0027] a. Accurately weigh 10 g of dried Tamarix multibranch stems and leaves, cut them into pieces and grind them, put them into an extractor, add 100ml of 70% ethanol solution for soaking, and use a Soxhlet extractor to extract once, each time for 1 hour, and the extraction temperature is up to To boiling temperature, discard the extract to remove small polar substances such as pigments, and dry the remaining stems and leaves to obtain the extract;

[0028] b. Add 100ml of pure water to the extract and boil for extraction, extract once, 3h each time;

[0029] c. Collect the cooled boiled extract, concentrate under reduced pressure at 60° C., and freeze-dry the stem and leaf extract of Tamarix multibranches.

[0030] Get the stem and leaf extract of Tamarix multibranches in Example 3, add distilled water to constant volume, and successively dilute and prepare the solution of Tamarix multibranches extract that the concentration is respectively 2, 1, 0.5, 0.25 mg / mL, and carry ...

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Abstract

The invention discloses a method for extracting an alpha- glucosidase activity inhibitor from plants, which uses whole plants as raw materials, obtains products without toxic and side effect, has high safety and low cost and is suitable to industrial production. The method comprises the following steps of: putting sheared and ground dry branchy tamarisk stem leaves or karelinia caspica stem leaves or reaumuria soongorica stem leaves in an extractor, adding an alcohol-water or acetone-water solution for soaking, wherein the ratio of the volume of the alcohol-water or acetone-water solution to the mass of the dry branchy tamarisk stem leaves or karelinia caspica stem leaves or reaumuria soongorica stem leaves is 8-30 ml / g of, and heating and extracting by backflow, wherein the extracting temperature reaches boiling temperature, the extracting time is 1-3, and the each extraction lasts 1.0-3.0 h; adding water for extracting for the second time, wherein the ratio of the volume of the water to the mass of the extractives is 8-30 ml / g, the extracting temperature reaches boiling temperature, extraction is carried out 1-3 times, and each extraction lasts1.0-3.0 h; collecting and mixing the cooled extractives, concentrating by pressure reduction at 60 DEG C, and finally freezing and drying to obtain the plant extractives.

Description

Technical field: [0001] The invention relates to an extraction method of an α-glucosidase activity inhibitor, in particular to a method for extracting an inhibitor of α-glucosidase activity, in particular to a method using plants (Tamina multibranch stems and leaves or Chai chinensis stems and leaves or Chai pipa stems and leaves) as raw materials, and the product has no toxic side effects and is safe A method for extracting α-glucosidase activity inhibitors from plants with high efficiency, low cost and industrial production. Background technique: [0002] Diabetes mellitus (DM) is a group of metabolic diseases with multiple etiologies, which is mainly related to the abnormal production and action of insulin. The syndrome of systemic and multiple organ damage is the main cause of cardiovascular and cerebrovascular diseases, blindness, amputation and renal failure in humans. At present, a variety of new hypoglycemic chemical drugs have been developed for clinical applicatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61K36/28A61P3/10
Inventor 谭成玉刘宇吴迪赵莹李倩
Owner DALIAN FISHERIES UNIVERSITY
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