Sheep blood high-activity immune globulin concentrate and preparation method thereof

A technology of immunoglobulin and high activity, applied in the field of food additives, can solve the problems of difficult food additive application, no detection of extraction of immunoglobulin, etc., and achieve the effect of simple method, low energy consumption and high activity

Inactive Publication Date: 2010-07-07
辽源市麒鸣生物技术综合开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"The method for extracting immunoglobulin IgG from pig blood" (application number is: 200510018302.2) discloses the method for extracting immunoglobulin from pig blood, but also does not find the report of extracting immunoglobulin from sheep blood, mainly because Sheep blood has a smell of mutton, and its extract also has a smell of mutton, so it is difficult to be used as a food additive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 goat blood highly active immunoglobulin concentrate

[0025] (1) Add concentration to 500g sheep blood and be 20% trisodium citrate solution 10g, stir, centrifuge (TGL-16A whizzer at 5000g, Changsha Pingfan Instrument Co., Ltd.) 6min then, separate and obtain two-layer solution, The lower layer is sheep hemoglobin solution, the upper layer is sheep plasma protein solution, and the upper layer sheep plasma protein solution (175g) is collected.

[0026] (2) Place the obtained sheep plasma protein solution in a closed container, heat up to 65° C., and pump air with a vacuum pump. When the vacuum degree reaches 0.085 MPa, pump air for another 2 minutes to obtain a sheep plasma protein solution without mutton.

[0027] (3) Dilute the sheep plasma protein solution obtained in step (2) with water to a concentration of 5%, and ultrafilter three times through a 120ku filter membrane (LM-125, Beijing Xubang Membrane Equipment Co., Ltd.) to obtain t...

Embodiment 2

[0030] The preparation of embodiment 2 goat blood highly active immunoglobulin concentrate

[0031] (1) adding concentration to 1000g sheep blood is the trisodium citrate solution 22g of 20%, stirs, then at 4000g centrifugal (TGL-16A centrifuge, Changsha Pingfan Instrument Co., Ltd.) 8min, separates and obtains two-layer solution, The lower layer is sheep hemoglobin solution, the upper layer is sheep plasma protein solution, and the upper layer sheep plasma protein solution (370g) is collected.

[0032] (2) Place the obtained sheep plasma protein solution in an airtight container, heat up to 64° C., pump air with a vacuum pump, and then pump air for 3 minutes when the vacuum degree reaches 0.086 MPa, to obtain a sheep plasma protein solution without mutton.

[0033] (3) The goat plasma protein solution obtained in step (2) was diluted with water to a concentration of 6%, and ultrafiltered twice through a 130ku filter membrane (LM-125, Beijing Xubang Membrane Equipment Co., Ltd...

Embodiment 3

[0036] Example 3 Preparation of Sheep Blood Highly Active Immunoglobulin Concentrate

[0037] (1) Add concentration to 1000g sheep blood and be that 20% trisodium citrate solution 22g, then centrifuge (TGL-16A centrifuge, Changsha Pingfan Instrument Co., Ltd.) 6min under 5000g, be separated into two layers of solutions, the lower floor is Sheep hemoglobin solution, the upper layer is a sheep plasma protein solution, and the upper layer sheep plasma protein solution (365g) is taken.

[0038] (2) Place the obtained sheep plasma protein in a closed container, heat up to 64°C, pump air with a vacuum pump, and then pump air for another 4 minutes when the vacuum degree reaches 0.086 MPa, to obtain a sheep plasma protein solution without odor.

[0039] (3) The goat plasma protein solution obtained in step (2) was diluted with water to a concentration of 7%, and ultrafiltered twice through a 120ku filter membrane (LM-125, Beijing Xubang Membrane Equipment Co., Ltd.) to collect the ret...

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Abstract

The invention discloses a sheep blood high-activity immune globulin concentrate and a preparation method thereof, belonging to the field of a food additive. Trisodium citrate is added to sheep blood for centrifugation so as to obtain sheep plasma protein solution; air exhaust is carried out in a closed container so as to obtain the sheep plasma protein solution without sheep odor; the sheep plasma protein solution is diluted by water and is ultra-filtered by a filtering membrane, and then freeze drying is carried out on the sheep plasma protein solution, so as to obtain the sheep blood high-activity immune globulin concentrate. Sheep blood immune globulin has the characteristics of high activity, no sheep odor, good taste and safe using. The preparation method can achieve high yield, and every kilogram of sheep blood can prepare more than 30 kilograms of sheep blood immune globulin concentrate. The preparation method is simple as well as easy and needs low cost; moreover, the energy consumption can be reduced by more than 52%. The invention makes full use of the resource of the sheep blood and reduces environmental pollution.

Description

technical field [0001] The invention belongs to the field of food additives, and in particular relates to a goat blood highly active immunoglobulin concentrate and a preparation method thereof. Background technique [0002] Immunoglobulin (Immunoglobulin, Ig) is a globulin that has antibody activity and can specifically bind to the corresponding antigen. It is a protein commonly found in mammalian blood, tissue fluid, lymph fluid and in vitro secretion fluid. Immunoglobulin has important immune and physiological regulation functions in animals, and plays an important role in improving the health of infants, middle-aged and elderly people, and people with low immunity. It is widely used in the development of functional food raw materials. [0003] Animal blood contains 1.5-2% active immunoglobulin, which is a rich and cheap source of active immunoglobulin. There are many methods for extracting immunoglobulins from animal blood, commonly used are salting-out method, organic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/06A23L1/305A23L33/17
Inventor 苏岩薛可苏东明伏圣兴杨苏扬
Owner 辽源市麒鸣生物技术综合开发有限公司
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