Method for heavy ion irradiation mutagenesis of streptomyces avermitilis strains and culture of irradiation strains

A technology of Streptomyces avermanii and culture method, which is applied in the field of heavy ion 12C+6 radiation mutagenesis, and can solve problems affecting production scale, high production cost, and difficulties

Inactive Publication Date: 2010-07-07
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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Problems solved by technology

[0008] During the selection and breeding of Streptomyces avermanii strains, the fermentation titer of the strains has been significantly improved by repeated treatment with ultraviolet rays and nitrosoguanidine, from 10 micrograms / ml of wild strains to the current 4000 μg / ml, but it is very difficult to increase it, which seriously affects the production scale and the production cost remains high

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  • Method for heavy ion irradiation mutagenesis of streptomyces avermitilis strains and culture of irradiation strains
  • Method for heavy ion irradiation mutagenesis of streptomyces avermitilis strains and culture of irradiation strains
  • Method for heavy ion irradiation mutagenesis of streptomyces avermitilis strains and culture of irradiation strains

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Embodiment Construction

[0099] 1. Provided by a heavy ion accelerator 12 C +6 Ion beam radiation mutagenesis was carried out on the spore suspensions of two samples of the starting strain S. 70, 80Gy, conduct the first radiation mutagenesis, study the radiation dose and the law of positive mutant strains, adjust the radiation dose, and then conduct the second radiation mutagenesis.

[0100] 2. Gradiently dilute the above-mentioned treated spore suspension, accurately draw 0.2ml / plate of the spore suspension of a sample, pour it on the prepared separation plate medium, separate a single colony, obtain a high titer, and draw Optimal positive mutation rate and mutagen dose.

[0101] 3. Use the same method as above to separate the spore suspension of another sample of the bacterial species with the common separation medium, and carry out the comparison of the colony morphology and the statistics of the lethality.

[0102] The formula of the above slant medium and separation plate medium is: soluble st...

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Abstract

The invention relates to a method for the heavy ion irradiation mutagenesis of streptomyces avermitilis strains and the culture of irradiation strains, belonging to the field of microbe strain breeding. The fermentation titer of the streptomyces avermitilis strains achieves 4000 microgrammes / milliliter by the traditional mutagenesis method and is very difficult to enhance again. The method comprises the following steps of: carrying out mutagenesis by adopting 12C+6 heavy ion beam irradiation, wherein an irradiation dose is 40-70 Gy, and a preferred irradiation dose is 50 Gy; and screening direct mutation strains of a plating medium and a slant medium to obtain high-titer strains reaching the fermentation titer of 6000 microgrammes / milliliter. The invention has stable and fast mutation, easy mutation control and simple, convenient and easy operation of the method.

Description

technical field [0001] The invention relates to the field of microbial strain breeding, in particular to the radiation mutagenesis using heavy ions 12C+6. Background technique [0002] Excellent microbial strains are the foundation and key of the fermentation industry. In order to greatly improve the types, output and quality of fermentation industrial products, it is first necessary to select and breed production strains with excellent performance. At present, the commonly used fermenting microorganism strain selection and breeding technology, one is the conventional mutation breeding method, and the other is the molecular breeding method. Conventional mutation breeding methods use physical mutagenic factors such as ultraviolet light for mutagenic breeding, or chemical mutagenic factors such as diethyl sulfate (DES), nitrosoguanidine, lithium chloride, coumarin, etc. Breeding, or compound mutagenesis breeding using physical and chemical factors. Conventional breeding has ...

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Application Information

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IPC IPC(8): C12N15/01
Inventor 李文建王曙阳陈积红刘敬颉红梅
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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