Method for identifying sperm motility of siganus canaliculatus

Abstract

The method discloses a method for identifying the sperm motility of the siganus canaliculatus, which relates to fish sperm testing. The sperms of the siganus canaliculatus are collected, and a kit is used to test the activities of total ATPase, creatine kinase (CK), succinodehydrogenase (SDH) and lactic dehydrogenase (LDH) in the sperms; if the activity of the total ATPase is higher than 9.20 plus or minus 0.28U / ml, the activity of the CK is higher than 25.59 plus or minus 0.13U / ml, the activity of the SDH is higher than 18.33 plus or minus 2.52U / ml and the activity of the LDH is higher than 14828.66 plus or minus 866.56U / L, the motility is good; and if the activity of the total ATPase is lower than 4.18 plus or minus 0.81U / ml, the activity of the CK is lower than 17.87 plus or minus 0.56U / ml, the activity of the SDH is lower than 9.33 plus or minus 1.53U / ml and the activity of the LDH is lower than 11324.33 plus or minus 689.58U / L, the motility is poor.

Description

technical field [0001] The invention relates to the detection of sperm motility in aquaculture fish. Background technique [0002] In production practice, the vitality of fish sperm is one of the important indicators reflecting the fertilization rate. Therefore, objective and accurate evaluation of the vitality of fish sperm has very important theoretical significance and application value. When evaluating fish sperm motility, there are mainly the following methods at present: 1. Use ordinary microscopes to observe and record the activity status and movement time of sperm. This method is greatly affected by human factors and cannot objectively reflect the activity status of sperm; 2. Use fish sperm to directly carry out fertilization test, and count the fertilization rate, hatching rate, film emergence rate, etc. This method is more affected by external environmental factors, and the test cycle will be longer with the length of early development time of different fish Big c...

AI Technical Summary

Problems solved by technology

When evaluating fish sperm motility, there are mainly the following methods at present: 1. Use ordinary microscopes to observe and record the activity status and movement time of sperm. This method is greatly affected by human factors and cannot objectively reflect the activity status of sperm; 2. Use fish sperm to directly carry out fertilization test, and count the fertilization rate, hatching rate, film emergence rate, etc. This method is more affected by external environmental factors, and the test cycle will be longer with the length of early development time of different fish Big changes; 3. Use computer-aided analysis system (CASA) to analyze the movement rate and movement distance of sperm, etc. This method can objectively reflect the movement of sperm, but the equipment is relatively expensive