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Method for identifying sperm viability of large yellow croaker

A technology for sperm motility and large yellow croaker, applied in the measurement of color/spectral characteristics, etc., can solve the problem of expensive equipment and other problems

Inactive Publication Date: 2010-07-14
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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AI Technical Summary

Problems solved by technology

[0005] 3. Use computer-aided analysis system (CASA) to analyze the movement rate and movement distance of sperm. This method can objectively reflect the movement of sperm, but the equipment is expensive

Method used

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Embodiment Construction

[0009] In the breeding season of large yellow croaker, the present invention collects the sperm of large yellow croaker in batches, and takes 5 fish sperm in each batch, and adopts a special kit for fish (carry out according to the product operation instruction book disclosed by Nanjing Jiancheng Bioengineering Research Institute) The activities of total ATPase, creatine kinase (CK), succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH) in different batches of sperm were measured by ultraviolet spectrophotometer, and each sample was repeated three times. The average value, using statistical software to analyze and process the results, if the average activity of ATPase in sperm is higher than 60.16±5.88U / ml, the average activity of CK is higher than 11.91±0.76U / ml, and the average activity of SDH is higher than 51±5. 2.16U / ml, if the average activity of LDH is higher than 7806.44±110.11U / L, the sperm motility is good; if the average activity of ATPase in sperm is between...

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Abstract

In order to solve the technical problems, the invention provides a method for identifying sperm viability of a large yellow croaker, relates to fish sperm viability detection, and provides a kit and an ultraviolet spectrophotometer to collect sperm of the large yellow croaker. The method is characterized in that the kit detects the viability of related energy metabolism enzyme in sperm by using the ultraviolet spectrophotometer; in the sperm, if the viability of ATP enzyme is higher than 60.16+ / -5.88U / ml, the viability of CK is higher than 11.91+ / -0.76U / ml, the viability of SDH is higher than 51+ / -2.16U / ml, and the viability of LDH is higher than 7,806.44+ / -110.11U / L, the sperm has higher viability; and in the sperm, if the viability of the ATP enzyme is lower than 3.54+ / -0.37U / ml, the viability of the CK is lower than 10.22+ / -0.32U / ml, the viability of the SDH is lower than 31.5+ / -2.08U / ml, and the viability of the LDH is lower than 2,654.13+ / -70.06U / L, the sperm has low viability.

Description

technical field [0001] The invention relates to the detection of sperm motility in aquaculture fish. Background technique [0002] The quality of fish sperm motility is one of the important indicators reflecting the fertilization rate. Therefore, in production practice, objective and accurate evaluation of fish sperm motility has very important theoretical significance and application value. When evaluating fish sperm motility, there are mainly the following methods: [0003] 1. Use an ordinary microscope to observe and record the activity and time of the sperm. This method is greatly affected by human factors and cannot objectively reflect the activity of the sperm; [0004] 2. Use fish sperm to directly carry out fertilization test, and count the fertilization rate, hatching rate, film emergence rate, etc. This method is more affected by external environmental factors, and the test cycle will be longer with the length of early development time of different fish Big chang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
Inventor 黄晓荣章龙珍庄平江琪刘鉴毅张涛冯广朋赵峰闫文罡
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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