Method for identifying sperm viability of large yellow croaker

Abstract

In order to solve the technical problems, the invention provides a method for identifying sperm viability of a large yellow croaker, relates to fish sperm viability detection, and provides a kit and an ultraviolet spectrophotometer to collect sperm of the large yellow croaker. The method is characterized in that the kit detects the viability of related energy metabolism enzyme in sperm by using the ultraviolet spectrophotometer; in the sperm, if the viability of ATP enzyme is higher than 60.16+ / -5.88U / ml, the viability of CK is higher than 11.91+ / -0.76U / ml, the viability of SDH is higher than 51+ / -2.16U / ml, and the viability of LDH is higher than 7,806.44+ / -110.11U / L, the sperm has higher viability; and in the sperm, if the viability of the ATP enzyme is lower than 3.54+ / -0.37U / ml, the viability of the CK is lower than 10.22+ / -0.32U / ml, the viability of the SDH is lower than 31.5+ / -2.08U / ml, and the viability of the LDH is lower than 2,654.13+ / -70.06U / L, the sperm has low viability.

Description

technical field

[0001] The invention relates to the detection of sperm motility in aquaculture fish. Background technique

[0002] The quality of fish sperm motility is one of the important indicators reflecting the fertilization rate. Therefore, in production practice, objective and accurate evaluation of fish sperm motility has very important theoretical significance and application value. When evaluating fish sperm motility, there are mainly the following methods:

[0003] 1. Use an ordinary microscope to observe and record the activity and time of the sperm. This method is greatly affected by human factors and cannot objectively reflect the activity of the sperm;

[0004] 2. Use fish sperm to directly carry out fertilization test, and count the fertilization rate, hatching rate, film emergence rate, etc. This method is more affected by external environmental factors, and the test cycle will be longer with the length of early development time of different fish Big chang...