High-purity isomaltose hypgather and alcohol co-production preparation method

Abstract

The invention belongs to the field of food industries and discloses a high-purity isomaltose hypgather and alcohol co-production preparation method. The method comprises the following steps of: preparing high-purity glucose higher than 95 percent by compounding diastatic enzymes; through diastatic enzyme conversion, fermenting by using dry activity yeast to prepare superhigh-purity isomaltose hypgather (the total content of isomaltose, panose and isomaltotriose are not less than 70 percent, and the total content of isomaltose hypgather is not less than 90 percent); and meanwhile, co-producingalcohol of 18 percent (the recovery rate is not less than 90 percent). The method can realize the organic combination of the superhigh-purity isomaltose hypgather production and the alcohol production and has novel process, excellent product quality, reduced cost, economy and practicability.

Description

technical field

[0001] The invention belongs to the field of food industry and relates to a production process of isomalto-oligosaccharide, more specifically, relates to a process method for co-production of high-purity 90% isomalto-oligosaccharide and alcohol by using enzyme technology and fermentation technology. Background technique

[0002] Isomaltooligosaccharide is a functional polysaccharide composed of 2 to 10 glucose molecules, which is made from cornstarch through the action of microbial enzymes. Isomaltooligosaccharide can promote the development of bifidobacteria in the intestinal tract, inhibit the growth of harmful bacteria, stimulate intestinal peristalsis, shorten the residence time of feces in the intestinal tract, and greatly reduce the chance of harmful ingredients being absorbed by the intestinal tract. And prevent constipation, improve the absorption of mineral elements such as calcium and magnesium in the large intestine, and reduce the production of to...

Examples

Embodiment 1

[0031] (1) pulping and liquefaction

[0032] Take 100kg of cornstarch, adjust the concentration to 17.5°Be' with water, adjust the pH value to 6.0, add 45ml of high-temperature-resistant α-amylase, and carry out continuous liquefaction for 100 minutes, and the liquefaction DE value is 14%.

[0033] (2) Saccharification

[0034] Cool the liquefied solution to 59°C, adjust the pH to 4.9, add 80ml of compound glucoamylase (Genencor OPTIMAXVHP4060), maintain the temperature at 58-60°C, stir intermittently, and measure the composition after 20 hours of incubation, glucose 96%. Enzyme treatment.

[0035] (3) Filtration, concentration

[0036]The temperature of the saccharification solution was raised to 80°C, 1kg of activated carbon was added, stirred at constant temperature for 0.3h, plate and frame filtered, and the filtered sugar solution was concentrated to 65% in a plate evaporator.

[0037] (4) Conversion

[0038] Adjust the pH of the concentrated saccharification solution...

Embodiment 2

[0048] (1) pulping and liquefaction

[0049] Take 150kg of cornstarch, adjust the concentration to 17.5°Be' with water, adjust the pH value to 6.0, add 70ml of high-temperature-resistant α-amylase, and carry out continuous liquefaction for 95 minutes, and the liquefaction DE value is 13%.

[0050] (2) Saccharification

[0051] Cool the liquefied solution to 58°C, adjust the pH to 4.8, add 120ml of compound glucoamylase Genencor OPTIMAXVHP4060, maintain the temperature at 58-60°C, stir intermittently, and measure the enzyme inactivation treatment after 20 hours of incubation.

[0052] (3) Filtration, concentration

[0053] The saccharification solution was heated to 80°C, 1.5kg of activated carbon was added, stirred at constant temperature for 0.3h, plate and frame filtered, and the filtered sugar solution was concentrated to 65% in a plate evaporator.

[0054] (4) Conversion

[0055] Adjust the pH of the concentrated saccharification solution to 4.2, add 1.5L of glucoamylas...

Embodiment 3

[0065] (1) pulping and liquefaction

[0066] Take 150kg of cornstarch, adjust the concentration to 17.5°Be' with water, adjust the pH value to 5.9, add 70ml of high-temperature-resistant α-amylase, and carry out continuous liquefaction for 93 minutes, and the liquefaction DE value is 12%.

[0067] (2) Saccharification

[0068] Cool the liquefied solution to 58°C, adjust the pH to 4.8, add 120ml of transglycosidase produced by Japan Amano Co., Ltd., maintain the temperature at 58-60°C, stir intermittently, and measure the enzyme inactivation treatment after 20 hours of incubation.

[0069] (3) Filtration, concentration

[0070] The saccharification solution was heated to 80°C, 1.5kg of activated carbon was added, stirred at constant temperature for 0.3h, plate and frame filtered, and the filtered sugar solution was concentrated to 65% in a plate evaporator.

[0071] (4) Conversion

[0072] Adjust the pH of the concentrated saccharified solution to 4.2, add 1.5L glucoamylase ...

technical field

[0001] The invention belongs to the field of food industry and relates to a production process of isomalto-oligosaccharide, more specifically, relates to a process method for co-production of high-purity 90% isomalto-oligosaccharide and alcohol by using enzyme technology and fermentation technology. Background technique

[0002] Isomaltooligosaccharide is a functional polysaccharide composed of 2 to 10 glucose molecules, which is made from cornstarch through the action of microbial enzymes. Isomaltooligosaccharide can promote the development of bifidobacteria in the intestinal tract, inhibit the growth of harmful bacteria, stimulate intestinal peristalsis, shorten the residence time of feces in the intestinal tract, and greatly reduce the chance of harmful ingredients being absorbed by the intestinal tract. And prevent constipation, improve the absorption of mineral elements such as calcium and magnesium in the large intestine, and reduce the production of to...

Examples

Embodiment 1

[0031] (1) pulping and liquefaction

[0032] Take 100kg of cornstarch, adjust the concentration to 17.5°Be' with water, adjust the pH value to 6.0, add 45ml of high-temperature-resistant α-amylase, and carry out continuous liquefaction for 100 minutes, and the liquefaction DE value is 14%.

[0033] (2) Saccharification

[0034] Cool the liquefied solution to 59°C, adjust the pH to 4.9, add 80ml of compound glucoamylase (Genencor OPTIMAXVHP4060), maintain the temperature at 58-60°C, stir intermittently, and measure the composition after 20 hours of incubation, glucose 96%. Enzyme treatment.

[0035] (3) Filtration, concentration

[0036]The temperature of the saccharification solution was raised to 80°C, 1kg of activated carbon was added, stirred at constant temperature for 0.3h, plate and frame filtered, and the filtered sugar solution was concentrated to 65% in a plate evaporator.

[0037] (4) Conversion

[0038] Adjust the pH of the concentrated saccharification solution...

Embodiment 2

[0048] (1) pulping and liquefaction

[0049] Take 150kg of cornstarch, adjust the concentration to 17.5°Be' with water, adjust the pH value to 6.0, add 70ml of high-temperature-resistant α-amylase, and carry out continuous liquefaction for 95 minutes, and the liquefaction DE value is 13%.

[0050] (2) Saccharification

[0051] Cool the liquefied solution to 58°C, adjust the pH to 4.8, add 120ml of compound glucoamylase Genencor OPTIMAXVHP4060, maintain the temperature at 58-60°C, stir intermittently, and measure the enzyme inactivation treatment after 20 hours of incubation.

[0052] (3) Filtration, concentration

[0053] The saccharification solution was heated to 80°C, 1.5kg of activated carbon was added, stirred at constant temperature for 0.3h, plate and frame filtered, and the filtered sugar solution was concentrated to 65% in a plate evaporator.

[0054] (4) Conversion

[0055] Adjust the pH of the concentrated saccharification solution to 4.2, add 1.5L of glucoamylas...

Embodiment 3

[0065] (1) pulping and liquefaction

[0066] Take 150kg of cornstarch, adjust the concentration to 17.5°Be' with water, adjust the pH value to 5.9, add 70ml of high-temperature-resistant α-amylase, and carry out continuous liquefaction for 93 minutes, and the liquefaction DE value is 12%.

[0067] (2) Saccharification

[0068] Cool the liquefied solution to 58°C, adjust the pH to 4.8, add 120ml of transglycosidase produced by Japan Amano Co., Ltd., maintain the temperature at 58-60°C, stir intermittently, and measure the enzyme inactivation treatment after 20 hours of incubation.

[0069] (3) Filtration, concentration

[0070] The saccharification solution was heated to 80°C, 1.5kg of activated carbon was added, stirred at constant temperature for 0.3h, plate and frame filtered, and the filtered sugar solution was concentrated to 65% in a plate evaporator.

[0071] (4) Conversion

[0072] Adjust the pH of the concentrated saccharified solution to 4.2, add 1.5L glucoamylase ...