Rapid breeding method of senecio cruentus

Abstract

The invention discloses a rapid breeding method of senecio cruentus, belonging to the cultivation field of plant tissues. The method comprises the following steps of: (1) serving senecio cruentus seeds as explants; (2) cultivating cluster buds by induction; (3) cultivating strong seedlings; (4) cultivating calluses by induction; (5) cultivating the calluses by enrichment; (6) cultivating adventitious buds by differentiation; (7) cultivating adventitious roots by induction; and (8) hardening and transplanting seedlings, wherein a minimal medium adopted in each cultivation stage is an MS medium, and a growth regulating agent added in each cultivation stage is selected with optimum dosage and proportioning ratio after being tested by a orthogonal design. The senecio cruentus bred by the method has the advantages of strongly grown test-tube seedlings, high breeding coefficient and scale and industrial production, the average induced rooting rate reaches 94.68%, and the survival rate of transplantation reaches 100%.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a cineraria (Senecio cruentus) seed rapid propagation method in vitro, and belongs to the field of plant tissue culture. Background technique [0002] Cineraria (Senecio cruentus), also known as Thousand Days Lotus, Melon Leaf Lotus, is a perennial herb of the genus Senecio in the Compositae family (Compositea). Cucumber leaves, hence the name cineraria. Native to the Canary Islands of Spain, it likes cold and cold, not resistant to high temperature and frost, good fertilizer, likes loose and well-drained soil. The suitable growth temperature is 10°C to 20°C, above 25°C and below 10°C, the growth is slow, and the temperature below 0°C is very susceptible to freezing damage. [0003] The cineraria plants are densely clustered. When in full bloom, the flowers cover the whole plant, and the flowers are terminal. Most of the flower heads are aggregated into corymbs, and the inflores...

AI Technical Summary

Problems solved by technology

The germination rate of somatic embryos obtained by the direct induction method is low, and due to the lack of nutrition or the accumulation of toxic metabolites in the medium, the explants will stop growing, or even age, blacken and die, thereby reducing the regeneration rate of the explants. It is not conducive to the regeneration of isolated tissues and large-scale culture

[0010] Yu Xiaoying and others from Hunan Agricultural University carried out in vitro culture and rapid propagation research on the single-bud stem segments of 5 different flower and color mother plants of Cineraria as explants. The results showed that: 1) the explants from the mother plant M1 The induction rate of the plant in the A5 medium containing 0.1mg / LNAA+2mg / LBA is the highest, and the buds germinate early and the number is large; 2) In the subculture, within the range of 0-2.0mg / L, with the concentration of The increase of cineraria buds increases, but when the BA is 3.0mg / L, the explant stems and leaves gradually become thicker and brittle over time, and become callus-like; 3) C6 medium (1 / 2MS+0.5mg / LIBA+2% sucrose) is a more suitable medium for in vitro rooting culture of cineraria, the root induction rate is 100% when cultured to the 16th day, the average root number is 18, and the average root diameter is 0.09cm The transplanting survival rate of the test-tube plantlets from C6 can reach 95%, but this propagation method does not obtain regenerated plantlets through dedifferentiation process and redifferentiation (that is, there is no induction of callus and the acquisition of adventitious buds), and the stable operation of the regeneration system Poor sex, low reproductive coefficient, not conducive to industrial production, and cannot be used for molecular breeding research

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