Rapid breeding method of senecio cruentus

A cineraria, rapid technology, applied in the field of plant tissue culture, can solve the problems of unfavorable in vitro tissue regeneration large-scale culture, poor stability of regeneration system, low germination rate of somatic embryos, etc., and achieves the promotion of callus growth and regeneration. The effect of stable system and high transplanting survival rate
CN101810144BInactive Publication Date: 2012-08-01BEIJING FORESTRY UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING FORESTRY UNIVERSITY
Publication Date
2012-08-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a rapid breeding method of senecio cruentus, belonging to the cultivation field of plant tissues. The method comprises the following steps of: (1) serving senecio cruentus seeds as explants; (2) cultivating cluster buds by induction; (3) cultivating strong seedlings; (4) cultivating calluses by induction; (5) cultivating the calluses by enrichment; (6) cultivating adventitious buds by differentiation; (7) cultivating adventitious roots by induction; and (8) hardening and transplanting seedlings, wherein a minimal medium adopted in each cultivation stage is an MS medium, and a growth regulating agent added in each cultivation stage is selected with optimum dosage and proportioning ratio after being tested by a orthogonal design. The senecio cruentus bred by the method has the advantages of strongly grown test-tube seedlings, high breeding coefficient and scale and industrial production, the average induced rooting rate reaches 94.68%, and the survival rate of transplantation reaches 100%.
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Description

technical field

[0001] The invention relates to a plant tissue culture method, in particular to a cineraria (Senecio cruentus) seed rapid propagation method in vitro, and belongs to the field of plant tissue culture. Background technique

[0002] Cineraria (Senecio cruentus), also known as Thousand Days Lotus, Melon Leaf Lotus, is a perennial herb of the genus Senecio in the Compositae family (Compositea). Cucumber leaves, hence the name cineraria. Native to the Canary Islands of Spain, it likes cold and cold, not resistant to high temperature and frost, good fertilizer, likes loose and well-drained soil. The suitable growth temperature is 10°C to 20°C, above 25°C and below 10°C, the growth is slow, and the temperature below 0°C is very susceptible to freezing damage.

[0003] The cineraria plants are densely clustered. When in full bloom, the flowers cover the whole plant, and the flowers are terminal. Most of the flower heads are aggregated into corymbs, and the inflores...

Claims

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