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Isolated culture and plant regeneration method of silver chain

An in vitro culture, Robinia pseudoacacia technology, applied in the directions of plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve problems such as difficulty in meeting the needs of Robinia pseudoacacia reproduction and breeding, low induction rate, etc., and achieve high repeatability and reproduction coefficient. The effect of high, high germination rate

Inactive Publication Date: 2013-02-13
BEIJING FORESTRY UNIVERSITY
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  • Claims
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Problems solved by technology

The above-mentioned studies all use immature zygotic embryos 1-4 days after flowering as explants to induce culture, and the induction rate is low, the highest induction rate is only 12%, which is difficult to meet the needs of the propagation and breeding of Robinia pseudoacacia. Therefore, the establishment of Robinia pseudoacacia Efficient somatic embryogenesis system is imperative

Method used

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  • Isolated culture and plant regeneration method of silver chain
  • Isolated culture and plant regeneration method of silver chain
  • Isolated culture and plant regeneration method of silver chain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Test materials

[0053] 1, the present invention takes the well-growing diploid Robinia pseudoacacia seedling population of Beijing Yanqing Mijiabao Nursery as a resource for drawing materials, and chooses 10 mother trees that grow well, have straight stems, and have a high seed setting rate (the interval between each strain is greater than 50 meters), between June and September 2010, collect the zygotic embryos of different developmental stages of Robinia pseudoacacia in the 4th to 12th week after flowering as explant test materials, collect once every 10 days, and collect 500 pods each time , that is, collect pods 25, 35, 45, 55, 65, and 75 days after flowering, collect 500 pods each time, store the pods collected on the same day in an ice box at low temperature and bring them back to the laboratory, and then put them in a refrigerator at 4°C Refrigerate for later use.

[0054] 2. Plant growth regulator

[0055] The plant growth regulating substances used in the ...

Embodiment 2

[0087] Except for the somatic embryo induction culture process, the 6-BA used in the somatic embryo induction medium was 1.0 mg / L, and the NAA was 1.0 mg / L, the rest were the same as in Example 1, and the data analysis results are shown in Table 2.

[0088] Table 2 Somatic Embryo Induction Culture Results

[0089]

[0090]

Embodiment 3

[0092] Except that in the somatic embryo induction culture process, the 6-BA used in the somatic embryo induction medium was 0.1 mg / L, and the NAA was 0.1 mg / L, the rest were the same as in Example 1, and the data analysis results are shown in Table 3.

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Abstract

The invention discloses an isolated culture and plant regeneration method of silver chain, which is characterized in that: zygotic embryos with different embryo ages are adopted as an explant, mass spectrometry (MS) basic culture medium, 2-4-Morpholinoethanesulfonic acid (MES) 500mg / L, glutamine 250mg / L, caseinhydrolysate 500mg / L, rhodofix 0.1 to 1.0mg / L, Amino-6-benzyl adenine 0 to 1.0mg / L, cane sugar 30g / L and agar 6g / L are used as a body cell embryo induction culture medium, the silver chain somatic embryo can be obtained through the direct induction, and then the somatic embryo is cultured to be mature, sprouted and grown to obtain the regeneration plant. The induction rate of the somatic embryo in the method is high, and the average quantity of the somatic embryos of each explant reaches 5.4 to 10.8; and the sprouting rate and the transplanting survival rate of the somatic embryo are high, a great amount of excellent silver chain regenerated plants can be formed in a short term, and the method belongs to a simple and rapid technical system for factorized mass production of silver chain plants.

Description

technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for in vitro culture and plant regeneration of black locust, and belongs to the field of tissue culture of black locust. Background technique [0002] Black locust (Robinia pseudoacacia L.), also known as acacia, belongs to the genus Robinia L. of the subfamily Fabaceae (Leguminosae), a deciduous tree, native to the United States, and naturally distributed in the Appalachian Mountains in the eastern United States. .Mt.) and the Ozank Mountains (Ozank.Mt.), which were introduced into my country in the early 20th century and rapidly expanded and planted after the introduction, are the main timber forests and firewood forests in the middle and lower reaches of the Yellow River, the Huaihe River Basin, the Haihe River Basin, and the lower reaches of the Yangtze River. , water and soil conservation forests, seawall and river embankment protection forests, there are 4 billion...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李云习洋王欢孙宇涵孙鹏袁存权李允菲戴丽胡瑞阳
Owner BEIJING FORESTRY UNIVERSITY
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