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Method for deacidifying and refining high-acid value fish oil by using enzyme method

A high acid value, fish oil technology, applied in the direction of fat oil/fat refining, fat production, etc., can solve the problems of high energy consumption, large lipase consumption, high comprehensive cost, etc.

Active Publication Date: 2012-08-22
XINGYE ZHOUSHAN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Esterification and deacidification usually uses acid or enzyme as a catalyst to re-esterify free fatty acid and glycerol into glycerol ester. The acyl acceptor of this method usually chooses glycerol. Due to the slow reaction speed of free fatty acid and glycerol, it takes a lot of lipase, and the energy consumption of the reaction process is relatively large, and the current comprehensive cost is still high, so this method has not been applied to production

Method used

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  • Method for deacidifying and refining high-acid value fish oil by using enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Such as figure 1 As shown, the acid value is that 5.0kg of tuna oil of 36.3mgKOH / g and dehydrated alcohol 90g join in the stainless steel material tank 2 (the stirrer in the material tank 2 is driven by stirring motor 1) with jacket, the packed bed enzyme reaction 50g immobilized enzyme novozyme 435 is loaded into device 4 (the inlet of packed bed enzyme reactor is provided with pressure gauge 6), and the mixture of high acid value tuna oil and dehydrated alcohol flows through enzyme reactor through gear material pump 3, and material The flow rate of the pump is 0.1L / min, so that the residence time of the reactant through the immobilized lipase is 60 seconds, and the reaction temperature is 60°C. During the reaction, samples are continuously taken through the sampling port 5 located at the outlet of the packed bed enzyme reactor. After removing ethanol by rotary film evaporator, analyze the changes of acid value and other components. After 6 hours, the acid value of oil...

Embodiment 2

[0033] Reactor such as figure 1 , 5.0kg of tuna oil with an acid value of 36.3mgKOH / g and 180g of absolute ethanol were added in a stainless steel material tank with a jacket, and 500g of immobilized enzyme novozyme 435, high acid value tuna oil and The mixture of absolute ethanol is transported through the enzyme reactor through the gear pump, the flow rate of the pump is 1L / min, the residence time of the reactant through the immobilized lipase is 60 seconds, the reaction temperature is 50°C, and samples are taken continuously during the reaction. After the sample is removed from ethanol by a rotary film evaporator, analyze the changes in acid value and other components. After 1 hour of enzyme reaction, the acid value of the oil becomes 4.7mgKOH / g, stop the reaction, recover ethanol, and take 1kg of oil after recovery of ethanol Add 38mL of NaOH solution and 150mL of distilled water with a mass concentration of 10%, stir while adding, a large amount of soap is produced, after...

Embodiment 3

[0035] Reactor such as figure 1 As shown, 5.0kg of tuna oil with an acid value of 36.3mgKOH / g and 120g of absolute ethanol were added to a stainless steel material tank with a jacket, and 250g of immobilized enzyme novozyme 435 was loaded into the packed bed enzyme reactor, and high acid value tuna The mixture of oil and absolute ethanol is transported through the enzyme reactor by a gear pump, the flow rate of the pump is 1L / min, the residence time of the reactant after passing through the immobilized lipase is 30 seconds, the reaction temperature is 40°C, and samples are taken continuously during the reaction , the sample was removed from ethanol by a rotary film evaporator to analyze the changes in acid value and other components. After 1 hour, the acid value of the oil became 6.2mgKOH / g, stop the reaction, recover ethanol, and add 1kg of oil after recovering ethanol Mass concentration is 10% NaOH solution 50mL and 150mL distilled water, stir while adding, a large amount of...

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Abstract

The invention discloses a method for deacidifying and refining high-acid value fish oil by using an enzyme method, comprising the following steps of: firstly, selectively esterifying free fatty acid reacting with alcohol in the fish oil by adopting immobilized lipase as a catalyst, and then neutralizing to obtain the refined fish oil. Compared with the traditional chemical refining method, because the method greatly reduces the acid value of grease, the refining yield is greatly improved, and the method has better economical efficiency and environmental protection feature.

Description

technical field [0001] The invention relates to a refining method of high-acid-value fish oil, in particular to an enzymatic deacidification and refining method of high-acid-value fish oil. Background technique [0002] Fish oil is rich in omega 3 fatty acids, especially two important functional fatty acids EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid). EPA is known as "vascular scavenger", which has the function of dredging and cleaning the heart blood vessels, thereby preventing various cardiovascular diseases. In addition to the function similar to EPA, DHA is also an indispensable material basis for the formation, development and operation of brain cells. At the same time, it also helps to activate weakened retinal cells, thereby nourishing the brain, improving vision, and preventing myopia. Fish oil is an important functional oil. [0003] Most fish oils are by-products of fishmeal processing. For large-scale fishmeal factories that use fresh aquatic prod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C11B3/02
Inventor 马永钧杨博范海星劳敏军王卫飞唐峰潘志杰
Owner XINGYE ZHOUSHAN
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