Extraction method of reactive black 5 dye efficient decolorizing bacteria

An extraction method and active black technology, applied in the field of separation and purification of microorganisms and bacteria, can solve the problem of less microorganisms, achieve high removal rate, decolorization promotion, and easy separation and purification methods

Inactive Publication Date: 2010-09-29
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there have been many reports on the degradation of dyes by microorganism

Method used

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  • Extraction method of reactive black 5 dye efficient decolorizing bacteria
  • Extraction method of reactive black 5 dye efficient decolorizing bacteria
  • Extraction method of reactive black 5 dye efficient decolorizing bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] (1) 5ml of activated sludge taken from the laboratory AAO was inoculated into 100ml of inorganic salt medium, where the inorganic medium was KH 2 PO 4 1.8g / L, Na 2 HPO 4 ·12H 2 O 3.5g / L, MgSO 4 ·7H 2 O 0.2g / L, FeCl 3 ·6H 2 O 0.01g / L, glucose 5.0g / L, distilled water 1000mL, pH=6.8~7.0. Use gradient pressure acclimation method to acclimate, add Reactive Black 5 dyes successively to make the concentration 10, 30 and 50 mg / L, change the concentration at an interval of 10 days, acclimate and cultivate for 2 months under static conditions at 30°C;

[0063] (2) Suction 5ml of sludge and add it to a 250ml Erlenmeyer flask containing 100ml of enriched culture solution, and anaerobic culture in a biochemical incubator at 30°C. Replace the culture solution every 5 days, that is, take out 10ml of the original culture solution and add 100ml fresh In the culture medium, every time the culture medium is replaced for the second time, the concentration of the dye in the enriched culture med...

Embodiment 2

[0067] (1) Take 1 mL of the bacterial liquid extracted in Example 1 and add it to 100 mL of the enriched culture solution (same as above), and incubate at 30°C for 12 hours. Add 5mL of the enriched bacteria solution to two 250mL conical flasks containing 100mL (active black 5 concentration of 50mg / L) enriched culture solution, respectively, and place them in a constant temperature incubator at 30°C and 30°C, 150r. In a shaker / min, the culture time is 35 hours. After culturing, observe the growth of bacteria and determine the remaining concentration of Reactive Black 5 in the solution through an electron microscope, so as to determine the strains with high efficient decolorization of Reactive Black 5.

[0068] (2) Take 1 mL of the bacterial solution extracted in Example 1 and add it to 100 mL of the enriched culture solution (same as above), and incubate at 30°C for 12 hours. Add 5 mL of the enriched bacteria solution to a 250 mL Erlenmeyer flask containing 100 mL (active black 5 ...

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Abstract

The invention relates to an extraction method of reactive black 5 dye efficient decolorizing bacteria, which comprises the steps of taking activated sludge and inoculating the activated sludge in an inorganic salt culture medium to carry out gradient pressure type domestication for 2 months, then inoculating the domesticated sample in the enrichment culture solution in the access amount of 5% for anaerobic cultivation at the temperature of 30 DEG C, simultaneously increasing the concentration of dye in the culture solution, finally carrying out separation and purification on the solid culture medium to ensure the growth of a single pure bacterial strain, and storing in a refrigerator of 4 DEG C after adding 50 wt% of glycerol for standby use. The method extracts active black 5 decolorizing bacterial strain from anaerobic activated sludge of an AAO system, the seed source is easily obtained and separated and purified simply and quickly, and the method has low cost and is environmental-friendly. The decolorization rate of the extracted bacterial strain on the reactive black 5 can reach 90 .48%, so the method has good application prospects.

Description

Technical field [0001] The invention belongs to the field of separation and purification of microbial bacteria, and particularly relates to an extraction method for reactive black 5 dye high-efficiency decolorizing bacteria. Background technique [0002] The global annual dye production has exceeded 7×10 5 t, where the azo dye contains one or more azo bonds (-N=N-), accounting for about 60% to 70%. Azo dyes are widely used as colorants because of their wide shade, high fastness, convenient application, bright color and low energy consumption. However, up to 50% of the dye is lost in the dyeing process. These colored wastewaters not only cause visual unpleasantness, but also the aromatic amines produced by their degradation have potential mutagenicity and carcinogenicity. [0003] There are physical, chemical and biological methods for the disposal of dye wastewater. Although physical and chemical methods such as coagulation, precipitation, adsorption and ozone oxidation are used...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
Inventor 陈亮陈刚陈东辉黄满红陈晔韩晓芳李俊生刘鑫
Owner DONGHUA UNIV
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