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Molecular marker closely related to rape humidity resistance, preparation method and application thereof

A molecular marker and moisture-resistant technology, which is applied in the field of rapeseed breeding and molecular biology, can solve the problems of long cycle, heavy workload of moisture damage identification, and easy to be affected by the environment, so as to improve the level of moisture resistance and reduce the workload of identification. The effect of accelerating the pace of genetic improvement

Inactive Publication Date: 2010-09-29
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a molecular marker closely related to moisture resistance of rapeseed, which can be used to carry out auxiliary breeding for moisture resistance, and solves the problems of large workload, long cycle and easy identification of moisture damage in conventional breeding methods. Affected by the environment and other issues, the pace of genetic improvement of rape resistance to humidity has been improved

Method used

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  • Molecular marker closely related to rape humidity resistance, preparation method and application thereof
  • Molecular marker closely related to rape humidity resistance, preparation method and application thereof
  • Molecular marker closely related to rape humidity resistance, preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0023] Example 1: Acquisition of SSR markers related to moisture resistance in rapeseed

[0024] In order to better understand the present invention, the present invention constructs a F 2 Isolate populations detailing methods to obtain molecular markers associated with moisture resistance. However, it should be understood that the above examples do not constitute any limitation to the present invention. In the following experimental steps, unless otherwise specified, all operations were carried out in accordance with the methods provided in "Molecular Cloning Experiment Guide" (Third Edition) (translated by Huang Peitang et al., Beijing: Science Press, 2002). The specific method is as follows:

[0025] (1) Group construction:

[0026] Using rapeseed variety Zhongshuang 9 with strong moisture tolerance (the relative vigor index of moisture tolerance is 0.87, a known and public variety) as the female parent, the rapeseed breeding material GH01 (relative vigor index of moistu...

Embodiment 2

[0063] Example 2: Identification effect of SSR markers related to humidity resistance in rapeseed

[0064] The application of a molecular marker closely related to moisture tolerance and resistance of rapeseed in improving the breeding of rapeseed moisture tolerance comprises the following steps:

[0065] (1) Population construction, DNA extraction and SSR marker analysis

[0066] The population construction, moisture resistance identification, DNA extraction and SSR marker analysis are the same as in Example 1, please refer to steps (1) to (4) for details. Refer to step (6) for the primer sequence used for the specific marker BnGMS130-350, and the size of the specific PCR fragment amplified by the marker is 350 bp.

[0067] (2) The identification effect of the specific marker BnGMS130-350 on the moisture resistance of rapeseed

[0068] with F 2 The 100 individual plants used for association analysis in the population were the research materials, and the average relative vi...

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Abstract

The invention discloses a molecular marker closely related to rape humidity resistance, a preparation method and application thereof. The preparation method comprises the following steps: (1) taking Zhongshuang No.9 and GH01 greatly different in humidity resistance as parents and obtaining an F2 colony through hybridization and bagging selfing in turn; (2) using a CTAB method to extract DNA of the parents and single-plant leaves of the F2 colony and adopting primers to perform PCR amplification and electrophoresis detection to obtain molecular-marker data; (3) using a seedling-stage anaerobic method to determine the index of seedling-stage humidity resistance of F2 single-plant seeds; and (4) performing association analysis on the molecular-marker data and the humidity-resistance index of the colony by using a multiple linear regression module in statistical software, namely SPSS software, and screening according to standards of a regression significance P of less than 0.05 and a contribution rate of more than 10 percent to obtain the molecular marker BnGMS130-350. The method is easy to implement and simple to operate, solves the problems that conventional breeding methods are high in workload of identifying moisture damage, long in cycle, susceptible to environment, and the like, and effectively speed up the steps of the genetic improvement on rape humidity resistance.

Description

technical field [0001] The invention belongs to the fields of rapeseed breeding and molecular biology, and more specifically relates to a molecular marker closely related to moisture resistance of rapeseed, and also relates to a preparation method of a molecular marker closely related to moisture resistance of rapeseed, and also relates to a rapeseed Application of Molecular Markers Closely Related to Moisture Tolerance and Resistance in Rapeseed Breeding for Moisture Tolerance and Resistance. Background technique [0002] Rapeseed is the most important winter oil crop in my country, and the Yangtze River Basin is the largest rapeseed producing area in my country, accounting for more than 80% of the national rapeseed area. However, the rice-rape crop rotation system is generally adopted in the Yangtze River Basin, and the climate is humid and rainy in autumn and spring, resulting in high groundwater levels in rice fields and serious wet damage to rapeseed. The perennial area...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陆光远张学昆程勇郑普英
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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