Method for producing cellulosic ethanol
A technology of cellulosic ethanol and cellulose-degrading bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high production cost and high investment cost of cellulosic ethanol
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
preparation example Construction
[0024] 1) Preparation of fermented seed liquid. The seed liquid culture medium uses urea as the nitrogen source, Thermoanaerobacter brackii uses 5g / L microcrystalline cellulose as the carbon source, and Thermoanaerobacter marsoni uses 5g / L glucose as the carbon source, and the rest of the ingredients are the same as those in 2 The fermentation medium formula is the same.
[0025] 2) According to the formula: KH 2 PO 4 1.5g / L, K 2 HPO 4 (anhydrous) 2.9g / L, nitrogen source 2.1g / L, MgCl 2 ·6H 2 O 1.0g / L, CaCl 2 2H 2 O 150mg / L, FeSO 4 ·6H 2 O 1.25mg / L, Cysteinehydrochloride 1.0g / L, resazurin 2.0mg / L, carbon source 5.0g / L, Morpholinopropanesulfonic acid (MOPS) 10.0g / L (you can not add it when the pH is constant), yeast extract 6.0g / L L, Sodium citrate 2H 2 O 3.0g / L to prepare the fermentation medium and sterilize it. Among them, the carbon source is cellulosic biomass, and the nitrogen source is organic or inorganic nitrogen source; the pH is adjusted to 7.6 before ste...
Embodiment 1
[0030] Embodiment 1: 100ml anaerobic bottle fermentation
[0031] 1) Use a 1ml syringe to take 100μl of the glycerol tube strains of Thermoanaerobacterium brockii and Thermoanaerobacterium mathranii, and inoculate them in 5ml of seed medium (according to the formula KH 2 PO 4 1.5g / L, K 2 HPO 4 (anhydrous) 2.9g / L, urea 2.1g / L, MgCl 2 ·6H 2 O 1.0g / L, CaCl 2 2H 2 O 150mg / L, FeSO 4 ·6H 2 O 1.25mg / L, Cysteine hydrochloride 1.0g / L, resazurin 2.0mg / L, microcrystalline cellulose 5.0g / L, Morpholinopropane sulfonic acid 10.0g / L, Yeast extract 6.0g / L, Sodium citrate 2H 2 O 3.0g / L, pH 7.6) in an anaerobic bottle, cultured at a constant temperature of 60°C to activate the strains.
[0032] 2) Inoculate 3ml of activated thermoanaerobic bacilli solution and 1ml of thermoanaerobic bacteria solution into 30ml seed culture medium respectively, and cultivate them in a constant temperature incubator at 60°C for 24-48 hours to obtain the first grade seed liquid.
[0033] 3) According...
Embodiment 2
[0036] Embodiment 2: 5L fermentation tank fermentation
[0037] 1) According to the method described in Example 1, prepare 200ml of the first-class seed liquid of Thermoanaerobacter bacillus marii, and 100ml of the first-class seed liquid of Thermoanaerobacter marsoni, and calculate the inoculum size to be 1: 10, and the total volume of the fermented liquid is The respective required volumes at 2L.
[0038] 2) According to the recipe [KH 2 PO 4 1.5g / L, K 2 HPO 4 (anhydrous) 2.9g / L, urea 17.8g / L, MgCl 2 ·6H 2 O 1.0g / L, CaCl 2 2H 2 O 150mg / L, FeSO 4 ·6H 2 O 1.25mg / L, Cysteinehydrochloride 1.0g / L, resazurin 2.0mg / L, microcrystalline cellulose 80g / L, Yeast extract 6.0g / L, Sodium citrate 2H 2 O 3.0g / L] to prepare a fermentation medium (calcium, magnesium, iron salt mother solution added after sterilization), 115 ° C, 15min damp heat sterilization.
[0039] 3) After the sterilization is finished, continuously feed nitrogen into the fermenter to remove the oxygen in the c...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More