Method for producing methanol by methane bioconversion

A technology of biotransformation and methane oxidizing bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve reliable technology, avoid unsafe hidden dangers, and strengthen the effect of gas-liquid mass transfer process

Inactive Publication Date: 2010-12-29
GRADUATE SCHOOL OF THE CHINESE ACAD OF SCI GSCAS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, methane-oxygen and methane-air mixtures are flammable and explosive, so there are still safety issues in the industrial production of methanol produced by aerobic bioconversion of methane

Method used

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  • Method for producing methanol by methane bioconversion
  • Method for producing methanol by methane bioconversion
  • Method for producing methanol by methane bioconversion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, free cell transforms methane to produce methanol

[0019] In 70ml anaerobic glass bottle (see attached figure 1 ) into 10ml of reaction solution containing 0.4g stem cells / L methanotrophic bacterium Methylosinus trichosporium OB3b (purchased from the American Type Microorganism Collection, numbered ATCC 35070) free cells in the reaction solution, 60mmol / L Na 2 HPO 4 -NaH 2 PO 4 Solution (phosphate concentration is 120mmol / L, pH 7), 20mmol / L sodium formate and 5mmol / L MgCl 2 , airtight, extract 30ml of air, then inject 30ml of methane, 30°C, 170rpm shaker shaker reaction for 17h, to generate 12mmol / L methanol solution.

Embodiment 2

[0020] Embodiment 2, free cell transforms methane to produce methanol

[0021] In 70ml anaerobic glass bottle (see attached figure 1 ) was added with 10ml of reaction solution containing 10g of stem cells / L methanotrophic bacteria Methylosinus trichosporium OB3b (purchased from the American Type Microorganism Collection, numbered ATCC 35070) free cells in the reaction solution, 200mmol / L Na 2 HPO 4 -NaH 2 PO 4 solution (phosphate concentration is 400mmol / L, pH 6), 20mmol / L sodium formate and 5mmol / L MgCl 2 , airtight, extract 10ml of air, then inject 10ml of methane, 30°C, 120rpm shaker shaker reaction for 20h, to generate 13mmol / L methanol solution.

Embodiment 3

[0022] Embodiment 3, immobilized cell transforms methane to produce methanol

[0023] In 70ml anaerobic glass bottle (see attached figure 1 ) was added with 10ml of reaction solution containing 20g of stem cells / L of methanotrophic bacteria Methylosinus trichosporium OB3b (purchased from the American Type Microorganism Collection, numbered ATCC 35070) polyurethane immobilized cells in the reaction solution, 600mmol / L Na 2 HPO 4 -NaH 2 PO 4 solution (phosphate concentration is 1200mmol / L, pH 6), 20mmol / L sodium formate and 5mmol / L MgCl 2 , airtight, extract 30ml of air, then inject 30ml of methane, 30°C, 120rpm shaker shaker reaction for 24h to generate 30mmol / L methanol solution.

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Abstract

The invention discloses a method for producing methanol by methane bioconversion. In the method, methylosinus trichosporium is used as biocatalyst, methanol containing gas and oxygen-containing gas are introduced into solution containing the methylosinus trichosporium and phosphate through a breathable film to react so as to prepare high-concentration methanol solution. The method realizes methanol production by methane bioconversion by using the high-concentration phosphate as methanol dehydrogenase inhibitor to inhibit further oxidation of the methane, realizes high-concentration methanol production by using high cell concentration methylosinus trichosporium as the biocatalyst, avoids unsafe hidden danger possibly generated by mixing of the methane and oxygen in a bubbleless film aeration mode, and reinforces the gas-liquid mass transfer process of the methane and the oxygen. By adopting the method, high concentration methanol solution can be safely and effectively produced, so the invention provides a reliable technology for industrialization of methanol production by methane bioconversion.

Description

technical field [0001] The invention relates to a method for producing methanol by converting methane, in particular to a safe and efficient method for producing methanol by bioconverting methane. Background technique [0002] With the increasing shortage of oil resources, how to effectively use the abundant natural gas resources to replace the lack of oil and solve the problem of difficult gas transportation has attracted worldwide attention. Methanol is one of the main chemical products of natural gas. It is not only a clean energy source, but also an important basic chemical raw material. Methane is the main component of natural resources and renewable resources such as natural gas, methane hydrate, biogas, and coal mine gas. Converting gaseous methane into liquid methanol can not only reduce transportation and storage costs, but also enable wider utilization of methane resources through methanol. Biogas is a renewable resource that can be produced by anaerobic fermenta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/04C12R1/01
Inventor 罗明芳段长虹江皓邢新会
Owner GRADUATE SCHOOL OF THE CHINESE ACAD OF SCI GSCAS
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