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Bloodstain detection method having long wavelength chemiluminescence and fluorescence, and developing functions

A chemiluminescence and detection method technology, applied in chemiluminescence/bioluminescence, fluorescence/phosphorescence, analysis by chemical reaction of materials, etc., can solve problems such as low visual sensitivity, short visual luminescence time, and difficulty in precise positioning of blood stains. , to achieve the effect of improving visual sensitivity and prolonging visual luminous time

Inactive Publication Date: 2012-07-04
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the defects of low visual sensitivity, short visual luminescence time and difficulty in precise positioning of blood stains in the above-mentioned detection method, and to provide a detection method with long luminescence wavelength, long luminescence time, and simultaneously has the functions of fluorescence and color rendering and precise positioning. Bloodstain detection method

Method used

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  • Bloodstain detection method having long wavelength chemiluminescence and fluorescence, and developing functions
  • Bloodstain detection method having long wavelength chemiluminescence and fluorescence, and developing functions
  • Bloodstain detection method having long wavelength chemiluminescence and fluorescence, and developing functions

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Effect test

Embodiment 1

[0038] Adopt the method that the present invention shows bloodstain form on printing paper as follows:

[0039] 1. Preparation of colorless fluorescein solution

[0040]Weigh 0.3333g of fluorescein into a 250mL beaker, add 7g of sodium hydroxide and 20g of distilled water, stir to dissolve, then add 10g of zinc powder, the mass ratio of fluorescein to sodium hydroxide, zinc powder, and distilled water is 1:21 : 30:60, put it in a boiling water bath, heat and stir until the green fluorescence fades away, place it in a dark place until the solution is clear, filter out zinc and zinc oxide residues, transfer to a volumetric flask, add distilled water, and prepare the concentration of the substance 1.0×10 -2 mol / L colorless fluorescein solution, add 1 to 3 zinc particles, and store in the refrigerator at 4°C.

[0041] 2. Preparation of luminol solution

[0042] Take by weighing luminol 0.4419g, be prepared with the sodium hydroxide solution that the amount concentration of subs...

Embodiment 2

[0056] Adopt the method that the present invention shows bloodstain form on printing paper as follows:

[0057] In this embodiment, the amount concentration of the substance is 1.0×10 -2 mol / L colorless fluorescein solution, the concentration of the substance is 1.0×10 -2 The luminol solution of mol / L, the sodium citrate aqueous solution that the substance concentration is 0.1mol / L, the disodium edetate aqueous solution that the substance concentration is 0.01mol / L, the substance concentration is 2mol / L The hydrogen peroxide aqueous solution of 1 L, double distilled water are mixed by following volume ratio:

[0058] Colorless fluorescein solution 1mL

[0059] Luminol solution 1mL

[0060] Sodium citrate aqueous solution 1mL

[0061] Disodium edetate aqueous solution 1mL

[0062] Hydrogen peroxide aqueous solution 1mL

[0063] Add double distilled water to 20mL

[0064] The pH value of the mixed solution was adjusted to 11.65 with a sodium hydroxide solution having a su...

Embodiment 3

[0067] Adopt the method that the present invention shows bloodstain form on printing paper as follows:

[0068] In this embodiment, the amount concentration of the substance is 1.0×10 -2 mol / L colorless fluorescein solution, the concentration of the substance is 1.0×10 -2 The luminol solution of mol / L, the sodium citrate aqueous solution that the substance concentration is 0.1mol / L, the disodium edetate aqueous solution that the substance concentration is 0.01mol / L, the substance concentration is 2mol / L The hydrogen peroxide aqueous solution of 1 L, double distilled water are mixed by following volume ratio:

[0069] Colorless fluorescein solution 4mL

[0070] Luminol solution 2mL

[0071] Sodium citrate aqueous solution 2mL

[0072] Disodium edetate aqueous solution 2mL

[0073] Hydrogen peroxide aqueous solution 10mL

[0074] Add double distilled water to 20mL

[0075] Adjust the pH value of the mixed solution to 11.65 with an aqueous sodium hydroxide solution with a ...

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Abstract

The invention discloses a bloodstain detection method having long wavelength chemiluminescence and fluorescence, and developing functions. The developing liquid of the method is prepared by mixing the water solutions of colourless fluorescein, luminol, hydrogen peroxide, sodium citrate and ethylene diamine tetraacetic acid. The visual sensitivity is enhanced and the visual luminescence time is prolonged by changing the chemiluminescence wavelength, meanwhile the stains with strong fluorescence are formed in the bloodstain area to develop the unsharp bloodstain shape. After the bloodstain search and the preliminary position are carried out by using the luminescence, the yellow-green fluorescence stain corresponding to the bloodstain shape can be observed on the bloodstain position detectedin preliminary by the luminescence method under the natural light, the lamplight or the ultraviolet light, then the following accurate position of the bloodstain by using the fluorescence and the vision is realized to facilitate the recording of the bloodstain shape and the sampling. The method can be popularized and used in the criminal cases investigation.

Description

technical field [0001] The invention belongs to the technical field of testing or analyzing materials by means of chemical properties of materials, and in particular relates to a bloodstain detection method with long-wavelength chemiluminescence, fluorescence and color development functions. Background technique [0002] Bloodstains are both traces and physical evidence, and the dual characteristics of bloodstains fully reflect its important position and role on the crime scene. As a trace, it can fully reflect the state of the victim when he was killed, the course of activities after the injury, and the trajectory of the criminal after touching the bloodstain. As one of the most important forensic evidence, the source identification of bloodstains can be realized by using the biological characteristics of bloodstains, that is, parameters such as bloodstain species, blood type, gender, and DNA. Although there have been many studies on on-site bloodstain search and location ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/76G01N33/72
Inventor 吕家根刘娜杨文静鲁芳
Owner SHAANXI NORMAL UNIV
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