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Method for inducing spawning of parent Solen grandis and collecting fertilized eggs

A technology for fertilized eggs and razor clams, which is applied in the field of artificially induced breeding of razor razor shellfish, can solve the problems that the two shells cannot be completely closed, the shells of large razor clam shells are thin and fragile, and the mortality rate of brood shellfish is high, so as to achieve good induction effect and the utilization of parents The effect of high rate and high seedling survival rate

Inactive Publication Date: 2012-08-15
INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the shells of large razor clam are thin and fragile, and the mortality rate of parent shellfish is high when they are caught many times. In addition, when the water pipes are stimulated when the big razor clams are caught, they will self-cut, which will affect their growth.
In addition, the mantle of the razor clam is open, the two shells cannot be completely closed, and it is easy to lose water after leaving the water. After being stimulated by drying for a long time, the survival rate of the broodstock is low.

Method used

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  • Method for inducing spawning of parent Solen grandis and collecting fertilized eggs
  • Method for inducing spawning of parent Solen grandis and collecting fertilized eggs
  • Method for inducing spawning of parent Solen grandis and collecting fertilized eggs

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Experimental program
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Effect test

Embodiment 1

[0027] Collection of shellfish: Begin to collect shellfish one after another from the first ten days of April. The shellfish is obtained by manual excavation. The surface of the shell should be smooth, the feet and appearance should be free of mechanical damage, healthy and vigorous, and the shell length should be 8-11cm;

[0028] Cultivation of broiler shellfish to promote maturation: broiler shellfish are placed in the oxytocin tank, which is required to be long and the bottom of the pool is inclined at an angle of 15° to the horizontal. The bottom of the pool is covered with 15cm thick coarse sand (0.5mm2 , change the water once a day, and the water change volume is 80%; the bait density is kept at 5-10×10 4 cell / ml, continuous aerated culture, the survival rate of temporary culture can reach more than 95%;

[0029] Oxygenation: Oxygenation can be initiated when the plumpness of the broodstock reaches over 85%, and the gonads are dissected and the sperm and eggs are self-flo...

Embodiment 2

[0032] The broodfish collected in late April were placed in the spawning pool for temporary raising, and the area of ​​the spawning pool was 12m 2 , the depth of the pool is 1.3 meters. After nearly 50 days of temporary cultivation, labor was induced on the morning of June 17, 2009. A small amount of eggs were laid at 8:20. When the fertilized eggs reached 15 / ml at 11:00, the siphon principle was used. Quickly move the fertilized egg into the cultivation pool 109#. The area of ​​each breeding pond is 23.8m 2 , 1.3 meters deep in the pond, the bottom of the cultivation pond is 60cm deeper than the bottom of the oxytocin pond. The fertilized eggs in the spawning pool can all be moved into the breeding pool. Immediately add sand and filter seawater to 109# quickly until the water level is 80cm, and the hatching density is 6 / ml.

[0033] After the ovum in the spawning pool was removed, the same method was used to add algae liquid (pH value 9.3) to the spawning pool, followed by...

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Abstract

The invention relates to a method for inducing the spawning of the parent Solen grandis and collecting fertilized eggs. The method comprises the following steps: the parent Solen grandis which has smooth surface of shells, no mechanical damage on feet and appearance, healthy body and high activity and also has the 8-11cm length of shells is collected to perform maturation and temporary cultivation in an induced spawning pond; when the meat condition of the parent Solen grandis is up to more than 85% and the genital gland is dissected to find that sperms / eggs can flow by gravity, the induced spawning can be performed, the liquid Isochrysis galbana extract is used to cover the entire bottom surface of the pond in the induced spawning process to stimulate the parent Solen grandis for 20-25min; when spawning is performed and the density of fertilized eggs in the induced spawning pond is up to 10-15 per milliliter, and the siphon principle is utilized to fast move the fertilized eggs to a culture pond to culture. The method of the invention has the advantages that the utilization rate of the parent Solen grandis is high, the induced spawning effect is good, the fertilized eggs are convenient and fast to collect, the fertilization rate is up to 72%, the breeding survival rate is high, etc. Only a small number of parent oysters can be used so as to obtain enough fertilized eggs for artificial breeding, thus the method has good application prospect.

Description

technical field [0001] The invention belongs to the field of artificial induced spawning and culture of brood clam broccoli, and in particular relates to a method for inducing spawning and collecting fertilized eggs of clam broccoli. Background technique [0002] Razor razor clams are large in size, high in meat yield, high in protein content, complete in amino acid types, tender and delicious in meat, and are important marine rare shellfish that are distributed along the coast of my country, but have few natural resources. At present, razor clam products rely on harvesting of natural resources, and the market price is 60-100 yuan / kg, which is 5-10 times the price of traditional cultured shellfish. Due to the drive of market interests, high-intensity fishing has made its resources show an obvious decline trend. Therefore, it is very important to carry out research on artificial breeding and multi-culture technology of razor clam and supply more razor clam commodities to the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 陈爱华姚国兴吴杨平张志伟张曹进吴建平
Owner INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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