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Biological prevention and control bacterial agent

A technology of bacterial agents and yeasts, applied in the direction of biocides, biocides, microorganisms, etc., can solve the problem of reducing

Inactive Publication Date: 2011-01-19
薛梦林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another biocontrol fungus of the genus Metschnikowia, M. fructicola, is isolated from the surface of grapes and has a certain control effect on grape gray mold (Botrytis rot) during storage. Using this biocontrol fungus 24 hours before harvest can significantly reduce grape storage. Diseases caused by pathogens B.cinerea, Alternaria spp. and Aspergillus niger, the exploration of the biological control mechanism of the antagonistic bacteria has not been reported

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0054] Example 1, Isolation and Identification of Metschnikowia Yeast

[0055] Alternaria alternate (Fr.) Keissler] is isolated from the surface of winter jujube with natural disease in the field. After purification, it is inoculated on PDA medium. Make spore suspension 1×10 6 spore ml -1 .

[0056] Dongzao is harvested in Wudi, Shandong Province, China, and Junzao is harvested in Jiaocheng, Shanxi Province, China. Choose fruits that are free from diseases and insect pests, harmless, with the same maturity and size, put them into PVC bags after pre-cooling for 2 days, and store them in a -2°C refrigerator. During the test, the jujube fruit hardness was 12.53kg / cm 2 , The soluble solid content is 36.8%, and the total acid is 0.46%. The hardness of Junzao is 12.65kg / cm 2 , The soluble solid content is 33.2%, and the total acid is 0.37%. Before inoculation, jujube fruits were sterilized with 2% NaOCl for 2 minutes, rinsed with distilled water, and air-dried for later use. ...

Embodiment 2

[0091] Embodiment 2, Metschnikowia zizyphicola sp.nov XY201 T Biocontrol agent

[0092] 1. Seed solution

[0093] Metschnikowia zizyphicola sp.nov XY201 T Inoculate in a Erlenmeyer flask filled with liquid medium, cultivate for 2 days, insert into a seed tank equipped with liquid medium, cultivate for 2 days, and obtain seed liquid.

[0094] The temperature of the seed liquid culture is 25°C, the initial pH of the liquid medium is 5.5, and the ventilation rate is 80mMO 2 l -1 h -1 .

[0095] Dissolve beef peptone, yeast extract, sucrose and NaCl in water in the above-mentioned liquid medium (triangle flask, seed tank culture medium), and fill up the volume with water, and the concentration of the beef peptone in the liquid medium is 1.5% (mass percentage), the concentration of the yeast extract in the liquid culture medium is 0.5% (mass percentage), the concentration of the sucrose in the liquid culture medium is 2.0% (mass percentage content), the concentration of the ...

Embodiment 3

[0110] Embodiment 3, Metschnikowia zizyphicola sp.nov XY201 T antibacterial test

[0111] 1. Metschnikowia zizyphicola sp.nov XY201 T Inhibition of Postharvest Diseases of Winter Jujube

[0112]The plant is winter jujube; the diseases to be controlled are black spot disease, rot disease, brown rot disease and penicillium disease. The pathogen of black spot disease is Alternaria alternate (Fr.) Keissler] (Wang Jun, Xin Yucheng. Isolation and identification of the pathogen of winter jujube black spot disease. "Journal of Qingdao Agricultural University (Natural Science Edition)", 2007 Year 1, 21-23., the public can get it from Xue Menglin.); the pathogenic bacteria of the rot disease is Fusarium decemcellulare Brick. (Geng Haifeng, Zhang Lizhen, Niu Wei. Screening and identification. Food Science, 2010, 31 (9): 150-155., the public can obtain from Xue Menglin.); the pathogen of Penicillium expansum (Lk.) Thom] (Liu Yang, Zhang Haiyu , Han Tao. Screening and identification of...

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Abstract

The invention discloses a biological prevention and control bacterial agent, and provides application of Metschnikowia zizyphicola sp. nov. XY201T to preventing and controlling plant diseases and application of the Metschnikowia zizyphicola sp. nov. XY201T to preparing a bacterial agent for preventing and controlling the plant diseases and refreshing harvested fruits and vegetables. The plant diseases comprise black spot, rot, gray mold, blue mold, soft rot, brown rot and green mold. The refreshing of the harvested fruits and vegetables is to inhibit the fruits and vegetables from rotting. Experiments prove that the separated Metschnikowia zizyphicola sp. nov. XY201T belongs to a new variety and has obvious effect of preventing and controlling the black spot of jujube, the rot of cucumber and eggplant and the like.

Description

technical field [0001] The invention relates to a biological control bacteria agent. Background technique [0002] Studies have shown that among the more than 20 species of Metschnikowia yeast found, there are no strains that have adverse effects on the human body and the environment. As a result, researchers have gradually become interested in the yeasts of this genus. The two strains found to have biocontrol effects abroad are Metschnikowia pulcherrima and Metschnikowia fructicola. M.pulcherrima is isolated from the surface of apples, and has certain inhibitory effects on postharvest pathogens Botrytis cinerea, Penicillium expansum, Alternaria sp. and Monilia sp. The suspension of this biocontrol bacterium can effectively inhibit the occurrence of diseases on apple wounds (in vivo), but its filtrate and sterilizing solution have no effect. It is inferred that this antagonistic bacterium does not produce antibiotics; on different solid medium ( In vitro) was cultured wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P3/00A23B7/155C12N1/16C12R1/645
Inventor 薛梦林
Owner 薛梦林
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