Application of BTG2 (B cell translocation gene 2) in preparing radiation sensitizing agent

A drug and gene technology, applied in the field of radiosensitization drugs, can solve the problems of BTG2 gene application that has not been reported, and achieve obvious synergistic effects of radiosensitization, increase sensitivity, and promote cell apoptosis

Inactive Publication Date: 2011-04-27
SUZHOU UNIV
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Problems solved by technology

[0007] However, the application of BTG2 gene in the preparation of radiosensitizers has not been reported at home and abroad.

Method used

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  • Application of BTG2 (B cell translocation gene 2) in preparing radiation sensitizing agent
  • Application of BTG2 (B cell translocation gene 2) in preparing radiation sensitizing agent
  • Application of BTG2 (B cell translocation gene 2) in preparing radiation sensitizing agent

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Embodiment Construction

[0027] The present invention will be further described below in conjunction with accompanying drawing and embodiment:

[0028] Materials: Human breast cancer cell lines MDA-MB-231 and MCF-7 were purchased from the American Cell Collection Center (ATCC), and were preserved and cultured by our laboratory; D-MEM medium dry powder, calf serum, and trypsin powder were purchased from Gibco company; standard protein molecular weight, SDS-PAGE loading buffer, RIPA protein lysate, TE electrophoresis buffer, 10× transfer membrane solution, 30% Acry-Bis, Tris-HCl, ammonium persulfate (AP), SDS, Tetramethylethylenediamine (TEMED), propidium iodide (PI) were purchased from Jiangsu Beyantian Biotechnology Research Institute; dimethyl sulfoxide (DMSO), agarose and Tween-20 were purchased from Shanghai Hi-tech Bioengineering Co., Ltd. ; Anti-BTG2, P53, Bax, cyclin D1, Ku-70, FEN-1 and XRCC1 antibodies were purchased from Santa Cruzs Biotechnology Company, USA. RNase A and 10 μg / mL proteinase...

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Abstract

The invention belongs to the field of radiation sensitizing agents, in particular to an application of BTG2 (B cell translocation gene 2) in preparing a tumor radiation sensitizing agent for ionizing radiation including X-rays. The invention discloses an application of liposome mediated eukaryotic expression plasmid pcDNA3-BTG2 in preparing the radiation sensitizing agent, wherein the liposome mediated eukaryotic expression plasmid pcDNA3-BTG2 is transfected to MCF-7 (macrophage chemotactic factor-7) breast cancer cells and MDA-MB-231 breast cancer cells and expressed successfully, and the BTG2 protein can up-regulate the proapoptosis Bax protein and down-regulate the cell cycle regulatory protein Cyclin D1, and by promoting the cell apoptosis, G0/G1 phase arrest of the cells is promoted, thereby increasing the sensitivity of the breast cancer cells to ionizing radiation including the X-rays.

Description

technical field [0001] The invention belongs to the field of radiotherapy sensitizing drugs, and in particular relates to the application of BTG2 gene (B cell migration gene 2) in the field of preparing radiotherapy sensitizing drugs. Background technique [0002] Breast cancer is one of the most common malignant tumors in women, and its incidence rate is 100 / 100,000 in European and American countries. The incidence of breast cancer has become the fastest-growing cancer in large and medium-sized cities in my country, and the age of onset is gradually becoming younger. It has become one of the diseases that seriously endanger the health of women in our country. Breast cancer treatment is currently dominated by surgery, supplemented by radiotherapy and chemotherapy according to pathological and clinical conditions after surgery. With the development of breast-conserving surgery, radiotherapy plays an even more important role in the treatment of breast cancer. [0003] Radiati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P35/00
Inventor 樊赛军胡旭东
Owner SUZHOU UNIV
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