Synthesis method for folate receptor targeted type nano gold particles

A technology of nano-gold particles and folic acid receptors, applied in the field of nano-gold particles, can solve the problems of insufficiency of particle morphology and inhomogeneous particle size, and achieve the effects of good cell compatibility, uniform particle size and enhanced stability.

Inactive Publication Date: 2011-04-27
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has the advantages of relatively mild synthesis conditions and better biocompatibility of the prepared gold nanoparticles, the shape of the particles is not regular enough, the particle size is not uniform enough, and the preparation takes a long time.

Method used

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  • Synthesis method for folate receptor targeted type nano gold particles
  • Synthesis method for folate receptor targeted type nano gold particles
  • Synthesis method for folate receptor targeted type nano gold particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take a clean 20mL beaker, add 10mL 1mmol / L HAuCl 4 solution, stirring and mixing at room temperature, and adding 1mL 1mmol / L folic acid solution dropwise to the solution to make HAuCl 4 The mass ratio to folic acid is 100:10, and then add 40 μl of 1mol / L NaOH solution to it until the solution is orange-yellow and clear, stir and mix at room temperature for 30 minutes, then immediately transfer it to a microwave reaction bottle, seal it, and the microwave reaction conditions are: 100°C, 120min, stirring speed 1200r / min. After the reaction, the reaction bottle was taken out, naturally cooled to room temperature, and dialyzed for 12 hours to remove unreacted small molecules, and the prepared samples were stored in a refrigerator at 4°C.

Embodiment 2

[0029] Take a clean 20mL beaker, add 10mL 1mmol / L HAuCl 4 solution, stirring and mixing at room temperature, and adding 0.9mL 1mmol / L folic acid solution dropwise to the solution to make HAuCl 4The mass ratio to folic acid is 100:9, then add 40 μl of 1mol / L NaOH solution to it until the solution is orange-yellow and clear, stir and mix at room temperature for 30 minutes, then immediately transfer it to a microwave reaction bottle, seal it, and the microwave reaction conditions are: 100°C, 120min, stirring speed 1200r / min. After the reaction, the reaction bottle was taken out, cooled naturally to room temperature, and dialyzed for 12 hours to remove unreacted small molecules to obtain folate receptor-targeted gold nanoparticles. The prepared samples were stored in a refrigerator at 4°C.

Embodiment 3

[0031] Take a clean 20mL beaker, add 10mL 1mmol / L HAuCl 4 solution, stirring and mixing at room temperature, and adding 0.8mL 1mmol / L folic acid solution dropwise to the solution to make HAuCl 4 The mass ratio to folic acid is 100:8, and then add 40 μl of 1mol / L NaOH solution to it until the solution is orange-yellow and clear, stir and mix at room temperature for 30 minutes, and immediately transfer it to a microwave reaction bottle, seal it, and the microwave reaction condition is 100 ℃, 120min, and the stirring speed is 1200r / min. After the reaction, the reaction bottle was taken out, cooled naturally to room temperature, and dialyzed for 12 hours to remove unreacted small molecules to obtain folate receptor-targeted gold nanoparticles. The prepared samples were stored in a refrigerator at 4°C.

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Abstract

The invention discloses a synthesis method for folate receptor targeted type nano gold particles, relating to nano gold particles and providing a synthesis method for folate receptor targeted type nano gold particles. The synthesis method is as follows: adding folate solution in HAuCl14 solution, mixing uniformly, then adding NaOH solution, and mixing uniformly, thus obtaining solution A; and carrying out microwave reaction on the solution A, cooling after reaction is ended, and dialyzing to remove small molecules unreacted, thus obtaining the folate receptor targeted type nano gold particles. By utilizing the synthesized folate receptor targeted type nano gold particles, tumor cells with the surface provided with high-level folate receptor expression can be specifically recognized, the targeting and cell compatibility are good, cells with the surface provided with high-level folate receptor expression can be specifically recognized, and the application in tumor cell detection is wide.

Description

technical field [0001] The invention relates to nano-gold particles, in particular to a synthesis method of folic acid receptor-targeted nano-gold particles. Background technique [0002] In recent years, biomacromolecules have been increasingly used as reducing agents for the preparation of gold nanoparticles (gold nanoparticles can be abbreviated as GNPs), including sugars, proteins, nucleic acids, etc. have been reported. Gold nanoparticles prepared from biological macromolecules have the characteristics of biological functionalization and are easy to combine with biological applications. The preparation process is usually non-toxic. Wang et al ([1] Li G P, Li D, Zhang L X, Zhai J F, Wang E K, One-Step Synthesis of Folic Acid Protected Gold Nanoparticles and Their Receptor-Mediated Intracellular Uptake [J] Chem.Eur.J., 2009, 15 : 9868-9873) reported a preparation of gold nanoparticles with folic acid as a reducing agent under mild conditions, and confirmed that the prepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B82Y40/00
Inventor 孙莉萍赖友群张召武马燕燕贾静
Owner XIAMEN UNIV
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