Method for preparing superparamagnetic polymer microspheres

Abstract

The invention provides a method for preparing monodispersed superparamagnetic polymer microspheres, which comprises the following steps of: swelling the polymer microspheres by adopting the swelling technology, and permeating oleic acid molecules into the polymer microspheres; raising the temperature, so that iron oleate is subjected to thermal decomposition in the microspheres to synthesize magnetic ferriferrous oxide nanoparticles, and the superparamagnetic polymer microspheres can be obtained. The prepared superparamagnetic polymer microspheres are uniform in grain diameter, controlled in magnetic content and stable in chemical properties, have the specific saturation magnetization of between 10 and 20 emu / g, and have potential application value in bioseparation.

Description

technical field

[0001] The invention relates to the technical field of polymer materials, in particular to a monodisperse magnetic polymer microsphere and a preparation method thereof. Background technique

[0002] As a new type of separation carrier, magnetic microspheres show unique advantages in bioseparation engineering and biomedical engineering, especially in cell separation, immobilized enzymes, immunoassays, targeted drugs, separation and purification of proteins and enzymes, etc. It has attractive application prospects in the application field. On the one hand, magnetic microspheres require that the microspheres have a high magnetic content, that is, a high specific saturation magnetization; at the same time, the particle size distribution of the microspheres is uniform, the performance is good, the preparation process is simple, the cost is low, and it is convenient for large-scale production. The methods for synthesizing magnetic polymer microspheres in the prior...

Examples

Embodiment 1

[0034] Take 10.8g FeCl 3 Add 36.5g of sodium oleate and 36.5g of sodium oleate into a 500ml flask, add 80ml of absolute ethanol and 140 n-hexane into the flask, after dissolving, heat at 70°C for 4h, wash the resulting black solution with deionized water for 3 to 4 times, and dry it in vacuum at 50°C Dry in the box for 24 hours to obtain hydrophobic iron oleate.

Embodiment 2

[0036] Take 16g of ferric sulfate and 37g of sodium oleate into a 500ml flask, add 80ml of absolute ethanol and 140 of n-hexane into the flask, after dissolving, heat at 70°C for 4h, wash the obtained black solution with deionized water 3 to 4 times, 50 °C in a vacuum oven for 24 hours to obtain hydrophobic iron oleate.

[0037] 2. Synthesis of polymer microspheres:

Embodiment 3

[0039] Synthesis of polystyrene microspheres by soap-free emulsion polymerization:

[0040] Add 620ml of deionized water and 1g of NaCl into the reactor, and after being dissolved, move it into a constant temperature water bath at 60°C, stir nitrogen for 10min, then add 65g of styrene monomer and 1.3g of crosslinking agent divinylbenzene, Continue stirring nitrogen for 30min. Then 0.65 g of potassium persulfate (KPS) was added, and after 24 h at 60° C., cooled to room temperature, the polymer emulsion was centrifuged to obtain polystyrene microspheres, which were washed repeatedly with deionized water. The product was dried under vacuum at room temperature for further use.

technical field

[0001] The invention relates to the technical field of polymer materials, in particular to a monodisperse magnetic polymer microsphere and a preparation method thereof. Background technique

[0002] As a new type of separation carrier, magnetic microspheres show unique advantages in bioseparation engineering and biomedical engineering, especially in cell separation, immobilized enzymes, immunoassays, targeted drugs, separation and purification of proteins and enzymes, etc. It has attractive application prospects in the application field. On the one hand, magnetic microspheres require that the microspheres have a high magnetic content, that is, a high specific saturation magnetization; at the same time, the particle size distribution of the microspheres is uniform, the performance is good, the preparation process is simple, the cost is low, and it is convenient for large-scale production. The methods for synthesizing magnetic polymer microspheres in the prior...

Examples

Embodiment 1

[0034] Take 10.8g FeCl 3 Add 36.5g of sodium oleate and 36.5g of sodium oleate into a 500ml flask, add 80ml of absolute ethanol and 140 n-hexane into the flask, after dissolving, heat at 70°C for 4h, wash the resulting black solution with deionized water for 3 to 4 times, and dry it in vacuum at 50°C Dry in the box for 24 hours to obtain hydrophobic iron oleate.

Embodiment 2

[0036] Take 16g of ferric sulfate and 37g of sodium oleate into a 500ml flask, add 80ml of absolute ethanol and 140 of n-hexane into the flask, after dissolving, heat at 70°C for 4h, wash the obtained black solution with deionized water 3 to 4 times, 50 °C in a vacuum oven for 24 hours to obtain hydrophobic iron oleate.

[0037] 2. Synthesis of polymer microspheres:

Embodiment 3

[0039] Synthesis of polystyrene microspheres by soap-free emulsion polymerization:

[0040] Add 620ml of deionized water and 1g of NaCl into the reactor, and after being dissolved, move it into a constant temperature water bath at 60°C, stir nitrogen for 10min, then add 65g of styrene monomer and 1.3g of crosslinking agent divinylbenzene, Continue stirring nitrogen for 30min. Then 0.65 g of potassium persulfate (KPS) was added, and after 24 h at 60° C., cooled to room temperature, the polymer emulsion was centrifuged to obtain polystyrene microspheres, which were washed repeatedly with deionized water. The product was dried under vacuum at room temperature for further use.

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