Method for measuring volatile fatty acid in fat tissue
A technology for volatile fatty acids and adipose tissue, which is used in measurement devices, preparation of test samples, instruments, etc., can solve the problems of complicated operation process, incomplete volatilization of volatile fatty acids, and large loss of volatile fatty acids.
Inactive Publication Date: 2013-03-20
NORTHWEST A & F UNIV
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Problems solved by technology
The first method can better simulate the cooking process of mutton, but the types of volatile substances produced are relatively complex, and the volatile fatty acids are not completely volatilized, so the quantitative aspect is limited to the relative content; the second method can use The complete volatilization of volatile fatty acids can be well quantitatively studied, but at present, the operation process is relatively complicated, and the loss of volatile fatty acids is relatively large
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[0027] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0028] 1 Materials and methods
[0029] 1.1 Materials
[0030] 1.1.1 Sample
[0031] The fat samples to be tested were collected from the core breeding area of sheep. Select 10 sheep around one year old. Take 200g of subcutaneous fat tissue from the waist of each sheep. All the fat samples are minced by a meat grinder, fully homogenized, wrapped in aluminum foil and stored in Store in the refrigerator at 20°C for later use.
[0032] 1.1.2 Main instruments
[0033] Equipped with the HP-6890 gas chromatograph (U.S. Hewlett-Packard Company) of hydrogen flame ion detector (FID);
[0034] HP6890-5973N Gas Chromatography-Mass Spectrometry (GC-MS), Hewlett-Packard Company, USA
[0035] 101-2AB electric blast drying oven, Tianjin Test Instrument Co., Ltd.
[0036] HH-4 digital display constant temperature water bath, Guohua Electric Co., Ltd....
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Abstract
The invention discloses a method for measuring volatile fatty acid in fat tissue, which comprises the following steps: A1: saponifying a fat tissue sample; A2: separating the volatile fatty acid from the saponified product obtained in the step A1 by using a distillation extraction device; A3: concentration; and A4: gas chromatography. Based on the prior art, in the invention, the distillation extraction and gas chromatography techniques are combined, the operation steps aiming at the feature of the volatile fatty acid are properly improved, the original fatty acid extraction process is omitted, gas chromatography is directly performed on the separated volatile fatty acid, thus the method has no derivatization; a set of simple, convenient and economic method for measuring volatile fatty acid in fat tissue is established which lays a foundation for deeply researching the volatile fatty acid in fat tissue.
Description
technical field [0001] The invention relates to a method for measuring volatile fatty acids in adipose tissue, in particular. Background technique [0002] Volatile fatty acids in adipose tissue play an important role in the flavor of meat produced during cooking, especially for ruminant meat. Since volatile fatty acids in animal adipose tissue have the characteristics of low content, strong volatility, and difficult operation, domestic research on fatty acids in animal adipose tissue mainly focuses on the range of long-chain fatty acids, while research on volatile fatty acids Very few, if any, studies on volatile branched-chain fatty acids have been reported. There are mainly two methods for the determination of volatile fatty acids in foreign research data: the first method is to heat the adipose tissue to the cooking temperature, and use a purge and trap device to absorb all volatile substances, and then at a higher Desorption is carried out at high temperature, and the...
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IPC IPC(8): G01N30/02G01N1/28G01N1/34
Inventor 陈玉林韩卫杰杨朝霞
Owner NORTHWEST A & F UNIV