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Toad peptide antibiotics for preparing medicine for treating liver cancer

A buadin antibiotic and a technology for treating liver cancer, which can be used in the field of preparing medicines for treating liver cancer, and can solve the problems of difficult product consistency and quality control.

Active Publication Date: 2012-11-07
黑龙江省蟾宝生物科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In principle, the aforementioned three traditional Chinese medicines can all be used to treat liver cancer. However, each prescription has a wide variety of medicinal materials. Due to differences in planting regions, climates, varieties, collection time, processing and processing methods, each medicinal material is not suitable for industrial production. When it is difficult to ensure product consistency and quality control

Method used

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  • Toad peptide antibiotics for preparing medicine for treating liver cancer

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Embodiment

[0010] 1. (1) Soak 1000g toad maggots in 5L-100L solution containing 1%-10% NaCl for 1h-10h, heat at 40°C-80°C and keep for 30min-300min, while stirring at 10r / min-36r / min to extract (2) Heat the above solution to boiling and keep it for 1min-10min, then rapidly cool it to room temperature, separate it with a tube separator at 16000r / min, remove the denatured protein, and wash the precipitate with 10L-20L distilled water Twice and separate with a tubular separator at 16000r / min, and collect the supernatant extract; (3) add the above-mentioned supernatant extract to the sephacryis-100HR column at room temperature, and then use pH5.1, 1mol / L The acetic acid buffer solution is used as gradient development and elution, and the flow rate is 0.1ml / min-1000ml / min to collect the eluent; (4) the bufodin antibiotic eluate collected is added to the mixture composed of n-butanol-ethyl acetate-water In the solvent system composed according to the ratio of 1.25:3.75:5, use ultrasonic standi...

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Abstract

The invention relates to a toad peptide antibiotics for preparing a medicine for treating liver cancer. The toad peptide antibiotics takes toad maggots as raw materials, and is extracted, separated and purified by technical processes of extracting by a solvent extraction method, removing protein by heating for denaturing, separating by gel chromatography columns, purifying by ultrasonic standing wave liquid phase preparative chromatography, drying by vacuum freeze drying and the like. The toad peptide antibiotics is used as an active component for treating liver cancer and combined with appropriate excipients to prepare medicines of various dosage forms, and the prepared medicines have definite components, stable and controllable quality and obvious curative effect on liver cancer. The invention has the advantages of reasonable production process and low energy consumption and is suitable for industrialized production.

Description

technical field [0001] The invention relates to the preparation of medicines for internal use, in particular to medicines for treating liver cancer prepared from buadin antibiotics. Background technique [0002] Liver cancer is a malignant tumor that occurs in the liver. Motherland's medical science thinks that the etiology mechanism of generation is, worry, anger is depressed, injures liver and spleen, and liver injury then liver-QI is not dredged, stagnation of QI and blood hematoma is hard. Injury to the spleen leads to poor spleen movement, poor water dampness, and ascites occurs when water damp stops gathering. It is generally believed that the onset of this disease is closely related to hepatitis and cirrhosis. [0003] CN94110216.6 discloses a series of traditional Chinese medicines for the treatment of liver cancer and its preparation method: it consists of Bupleurum chinensis, white peony root, three edges, curcuma, green bark, woody, poria cocos, citrus aurantium,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/00A61K9/127A61K9/00A61K9/16A61P35/00A61P1/16A61K35/64
Inventor 徐华民赵荣华
Owner 黑龙江省蟾宝生物科技发展有限公司