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Three-dimensional bone-like structure artificial bone with high bioactivity and high strength and preparation method thereof

A three-dimensional structure, biological activity technology, applied in the field of artificial bone, can solve the problems of lack of support, low biological activity, poor strength and so on

Active Publication Date: 2011-06-29
山东宇宁医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the problems of low bioactivity, poor strength and lack of support in the initial stage of implantation in existing bone-like materials, and to provide a three-dimensional bone-like structure with high bioactivity and high strength artificial bone

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  • Three-dimensional bone-like structure artificial bone with high bioactivity and high strength and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] (1) The three-dimensional structure natural bone is taken from the cancellous bone in the femur of the bovine bone. The sample size is 10mmx10mmx2mm (the size is the length, width and height of the cancellous bone). The aqueous solution, 30% hydrogen peroxide and 80vol% ethanol were soaked for 24 hours, and then sintered at 1050° C. for 12 hours to prepare the HA three-dimensional structure scaffold with a pore size of 300-500 μm.

[0022] (2) The HA three-dimensional structure scaffold was soaked in 10M NaOH aqueous solution for 96 hours.

[0023] (3) Dissolve 0.3mg of collagen-2 (rhBMP-2) in 100ml of 1.5 SBF body fluid to obtain bone-like solution;

[0024] (4) The HA scaffold treated in step (2) was soaked in the bone-like solution for 72 hours, and then vacuum-dried at room temperature for 4 hours to prepare a three-dimensional bone-like artificial bone with high bioactivity and high strength.

[0025] The HA three-dimensional osteoid structure artificial bone prep...

Embodiment 2

[0035] (1) Natural bone with three-dimensional structure is taken from the cancellous bone in the femur of bovine bone. The sample size is 10mmx10mmx2mm, and there are 10 pieces in total. Sintering at 1100° C. for 10 hours to prepare a HA three-dimensional structure scaffold with a pore size of 300-500 μm.

[0036] (2) The HA three-dimensional structure scaffold was soaked in 10M NaOH aqueous solution for 96 hours.

[0037] (3) Dissolve 0.5mg of collagen-2 (rhBMP-2) in 100ml of 1.5 SBF body fluid to obtain bone-like solution;

[0038] (4) The HA scaffold treated in step (2) was soaked in the bone-like solution for 124 hours, and then vacuum-dried at room temperature for 4 hours to prepare a three-dimensional bone-like artificial bone with high bioactivity and high strength.

[0039] The HA three-dimensional osteoid structure artificial bone prepared in this example was tested, and its compressive strength was: 7.26±0.25MPa;

[0040] The activity detection method of the artif...

Embodiment 3

[0043] (1) Natural bone with three-dimensional structure is taken from the cancellous bone in the femur of bovine bone. The sample size is 10mmx10mmx2mm, a total of 10 pieces, which are sequentially soaked in 10M sodium hydroxide aqueous solution, 30% hydrogen peroxide and 80-95vol% ethanol for 36 hours. Then sintered at 900° C. for 14 hours to prepare a HA three-dimensional structure scaffold with a pore size of 300-500 μm.

[0044] (2) The HA three-dimensional structure scaffold was soaked in 10M NaOH aqueous solution for 96 hours.

[0045] (3) Dissolve 0.1 mg of collagen-2 (rhBMP-2) in 100 ml of 1.5 SBF body fluid to obtain a bone-like solution;

[0046] (4) The HA scaffold treated in step (2) was soaked in the bone-like solution for 72 hours, and then vacuum-dried at room temperature for 4 hours to prepare a three-dimensional bone-like artificial bone with high bioactivity and high strength.

[0047] The HA three-dimensional osteoid structure artificial bone prepared in t...

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Abstract

The invention discloses a three-dimensional bone-like structure artificial bone with high bioactivity and high strength and a preparation method thereof. The preparation method comprises the following steps of: (1) performing mineralization treatment on a three-dimensional structure natural bone; (2) sintering the three-dimensional structure natural bone subjected to mineralization treatment to obtain a hydroxyapatite (HA) three-dimensional structure scaffold or a beta-tricalcium phosphate (TCP) three-dimensional structure scaffold; and (3) soaking the HA three-dimensional structure scaffold or the beta-TCP three-dimensional structure scaffold in biomimic bone solution, and performing vacuum pumping to obtain the artificial bone. In the preparation method, the three-dimensional structure natural bone is subjected to mineralization treatment and sintering, and then is compounded with simulated body fluid which has high bioactivity and contains collagen to form the three-dimensional structure HA or beta-TCP scaffold with high bioactivity and high strength; and the compression strength of the artificial bone is 5MPa, the porosity is between 70 and 75 percent, and the aperture is between 100 and 600mu m. The three-dimensional bone-like structure artificial bone with high bioactivity and high strength can repair various bone defects and induce osteogenesis.

Description

technical field [0001] The invention relates to artificial bone and a preparation method thereof, in particular to a preparation method of a three-dimensional bone-like structure artificial bone with high biological activity and high strength and a product prepared by the method, belonging to the field of artificial bone. Background technique [0002] At present, there are many patents on artificial bone and its preparation methods, including not only HA, β-TCP materials but also a large number of composite materials. Patents for HA or Nano-HA combined with collagen, such as US 7229971, US20170255422, US 200220018797, and JP 2003073304, etc., although they have bone fusion ability, their initial strength is poor and they cannot play a supporting role. They are useful for repairing some bone defects. difficulty. Contents of the invention [0003] The technical problem to be solved by the present invention is to overcome the problems of low bioactivity, poor strength and la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/28
Inventor 温宁田杰谟
Owner 山东宇宁医疗器械有限公司
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