Method for establishing cauliflower hybrid seed DNA (deoxyribonucleic acid) fingerprint spectrum and application thereof

Abstract

The invention discloses a method for establishing a cauliflower hybrid seed and a parent DNA (deoxyribonucleic acid) fingerprint spectrum and the application thereof. The establishment method comprises the following steps: 1) extracting and purifying cauliflower DNA; 2) using the obtained high-purity cauliflower DNA as a template for performing RAPD (random amplified polymorphic DNA) and SRAP (sequence-related amplified polymorphism) analysis; and 3) selecting selective polymorphism amplified bands to construct the DNA fingerprint spectrum of a tested material; and the cauliflower hybrid seed and parents thereof in the DNA fingerprint spectrum have specific DNA fingerprints which can be mutually differentiated. The DNA fingerprint spectrum is represented in the form of a diagram, thereby being relatively intuitive to look and being easy to understand; and the DNA fingerprint spectrum is converted to the digital representation form, thereby being convenient to be identified, read and analyzed by a computer. The method can be applied in identification of seed purity of the cauliflower hybrid seed, the result is accurate and reliable, and the detection is rapid.

Description

technical field [0001] The invention relates to cauliflower seed identification technology, in particular to a method for establishing DNA fingerprints of cauliflower hybrids and its application. Background technique [0002] At present, many varieties of cauliflower (Brassica oleracea L.var.botrytis L) popularized and applied in my country are the first generation of hybrids. The first generation of hybrids can synthesize many excellent characters of the parents, and the economic benefits are better. [0003] However, in the process of seed production, due to improper management measures and the defects of self-incompatibility itself, self-seeds, ie false hybrids, are often produced, resulting in a decrease in seed purity. Therefore, improving the purity of hybrids is an important topic in the field of heterosis utilization research. At present, there are roughly three methods for variety identification and seed purity analysis, namely field morphology, biochemical marker...

AI Technical Summary

Problems solved by technology

The traditional field identification method uses the color, shape and other agronomic traits of seeds, seedlings, leaves, flowers, fruits, pollen and other tissues and organs as the observation indicators for identification. Although it is a relatively feasible method in production practice, field identification requires Additional land occupation, long period, high cost, not only limited by seasons, but also the performance of many traits is affected by cultivation measures and environmental factors, and the observation experience of the appraiser also restricts the accuracy of appraisal

The biggest defect of Daejeon identification is that it cannot provide timely information on the purity of seeds, and cannot provide information on the quality of seeds before a large number of seeds are traded, and cannot play a role in pre-supervising the quality of seeds

Biochemical identification methods include protein electrophoresis and isoenzyme electrophoresis, which have been increasingly widely used, but they cannot fully display the polymorphisms between varieties, and it is difficult to effectively distinguish varieties with close relatives

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