Tetracycline medicament selective electrode and preparation method thereof

Abstract

The invention discloses a tetracycline medicament selective electrode and a preparation method thereof. The electrode comprises a quartz glass tube containing a molecularly imprinted monolithic column with an electroactive substance, an internal filling liquid, an internal reference electrode, an electrode stem and epoxy resin. By increasing the thickness of a sensitive film, using a porous monolithic column material, efficiently binding tetracycline medicaments in the electroactive substance to the sensitive film and optimizing the internal filling liquid, the detection limit of the electrode is reduced to the order of magnitude of 10<-9> mol / L. Because the molecularly imprinted monolithic column has a specific recognition action on the tetracycline medicaments, the selectivity is high, and common interfering substances have no influence on the potential measurement of the electrode. By using the tetracycline medicament selective electrode provided by the invention as an indicator electrode and a saturated calomel electrode or a Pt electrode as a reference electrode, the tetracycline medicaments can be detected with high sensitivity and high selectivity by using the potential analysis method, equipment is simple, the analysis cost is low, and the residual analysis of the tetracycline medicaments can be realized.

Description

technical field [0001] The invention relates to the technical fields of electroanalytical chemistry and chemical sensors, in particular to a preparation method of a tetracycline drug selective electrode. Background technique [0002] Tetracyclines (Tetracycline, TCs) are a class of broad-spectrum antibacterial drugs with a dodecahydrotetraphenylene structure, and are mainly used clinically to treat Gram-positive and negative bacteria, mycoplasma, chlamydia, rickettsia and spirochetes Infections caused by pathogens. TCs mainly include two categories, that is, natural tetracyclines include tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC), demeclocycline (DMC), etc., and semi-synthetic tetracyclines include methene Doxycycline (MTC), doxycycline (DC), minocycline (MNC), etc., among which the most commonly used TCs are TC, OTC, CTC, and DC. With the advantages of low price and wide antibacterial spectrum, it is widely used as a feed additive in the breeding in...

AI Technical Summary

Problems solved by technology

[0007] The primary technical problem to be solved by the present invention is to overcome the deficiencies of several methods for the above-mentioned existing detection of tetracycline drug content in samples, including: sample pretreatment is complicated, professional operators are needed, the instrument is expensive, the analysis cost is high, and the analysis speed is relatively high. Slow, long detection time, not suitable for on-site detection, poor stability and precision, high requirements on the sample itself, not suitable for colored, turbid, viscous liquids, prone to false positives, low sensitivity of the results (detection Limit > 3mg / kg), specificity is not strong, etc., providing a tetracycline drug selective electrode based on molecularly imprinted monolithic column, which has high sensitivity and selectivity, can directly detect colored solutions, and the detection speed is fast; precision High precision and accuracy; the instruments used are simple and the analysis cost is low

Images