Fusion tagging protein for nonchromatographic sepration of target protein and coding gene and preparation method thereof

Abstract

The invention discloses a fusion tagging protein for the nonchromatographic sepration of a target protein, which has an amino acid sequence in a sequence table list 1, or a fusion protein of the amino acid sequence, or a functional fragment of the amino acid sequence or a derivative of the functional fragment of the amino acid sequence. The invention also discloses the coding gene and preparation method of the fusion tagging protein. The fusion tag prepared by the invention can be connected with a target protein gene simply and quickly to realize high-efficiency expression; the structure and normal function of the target protein are hardly affected, and the safety is high; and the expression condition of the protein is simple, the purification is convenient and the production cost is low, so the fusion tagging protein is suitable to be produced industrially.

Description

technical field [0001] The invention relates to a fusion tag protein capable of performing non-chromatographic separation on a target protein, its coding gene and a preparation method. Background technique [0002] In recent years, with the rapid development of biotechnology, especially genetic engineering, protein expression has become easier. Relatively speaking, purification is a very complicated work. Large-scale separation and purification of protein molecules is a key technical issue in current bioengineering, and the cost of the purification process accounts for about 60%-70% of the total cost. The fusion tag technology that emerged at the end of the 20th century has promoted the development of recombinant protein purification technology. The main process is to use recombinant DNA technology to fuse a gene encoding a certain tag at the 3′ end or 5′ end of the target protein coding gene, and express the recombinant protein through the host. Protein, the recombinant p...

AI Technical Summary

Problems solved by technology

However, the cost of enzymatic hydrolysis is high (the price of these enzymes is generally quite expensive), the reaction time is long (more than 10 hours), and more importantly, these enzyme proteins are inevitably mixed into the target protein, causing new pollution, which needs to be added after lysis. Chromatographic separation and removal complicate the purification process

In addition, the high price of specific ligands combined with fusion tags also limits the application in large-scale separation and purification

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