Unlock instant, AI-driven research and patent intelligence for your innovation.

Device and method for detecting molecular interactions

A technology of target molecules and probe molecules, which is applied in the field of specific interaction devices, can solve the problems of reducing the detection signal, slowing down the detection method, etc., and achieves the effects of improving repeatability, avoiding pollution, and facilitating external intervention.

Active Publication Date: 2011-07-20
CLONDIAG GMBH
View PDF26 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, such an extra step slows down the detection method significantly
In addition, the detection signals of the detected nucleic acids that specifically hybridize to the nucleic acid probes on the microarray are also significantly reduced

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Device and method for detecting molecular interactions
  • Device and method for detecting molecular interactions
  • Device and method for detecting molecular interactions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0323] Example 1: Structure of a reaction chamber with an integrated heater

[0324] exist Figure 8 and Figure 9 An embodiment of a processing unit without an integrated heater and a device for directing a DNA chip towards a detection plane is described in . The DNA chip in the shown device can be read out by a conventional fluorescence microscope (eg Axioskop, Zeiss, Jena, Germany).

Embodiment 2

[0325] Example 2: Structure of a reaction chamber with a silicon heating substrate

[0326] Figure 10 and 11 The variant of the processing unit of the device of the invention shown in is a miniaturized reaction chamber containing an integrated probe array (DNA chip), a silicon heating substrate with integrated temperature sensors for regulating the different temperatures in the reaction chamber ("heating substrate"), and optionally a circuit board with EPROM for making electrical contact with the heating substrate. Each element is embedded in two shells made of synthetic material. The entire unit is a spatially closed system in which all required reactions (eg PCR) can be performed in a temperature-controlled manner.

[0327] First insert the board into the posts provided in the lower case (with the EPROM facing down). On the upper side of the circuit board, three electrical contact pads are arranged, which ensure the electrical connection to the subsequently inserted h...

Embodiment 3

[0334] Example 3: Detection of background signal reduction by dislocation of analyte

[0335] All fluorescence measurements described in this example were performed by a fluorescence microscope (Zeiss, Jena, Germany). Excitation was performed with incident light using a white light source, and filters were set to suit cyanine 3. Signals were recorded by a CCD camera (PCO-Sensicam, Kehlheim, Germany). In the underside, the thickness of the slit represents the distance between the microarray and the detection plane.

[0336] a) Measure the fluorescence signal of the analyte according to the thickness of the slit

[0337] Channel shells with defined channel depths (5m, 10m, 28m) were cast by Sylgard. The width of the channel is 125 μm. Glass chips are placed in channels of varying depths. The channel was then filled with a solution of 200 nM Cy-3 labeled oligonucleotide in 2x SSC + 0.2% SDS and the signal was measured using an exposure time of 1.5 seconds.

[0338] exist ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention especially relates to a device for the qualitative and / or quantitative detection of molecular interactions between probe and target molecules, which comprises a) a microarray comprising a substrate on which probe molecules are immobilized on array elements, said microarray being located on a first surface of the device; and b) a reaction chamber that is defined between the first area with the microarray disposed thereon and a second surface, the distance between the microarray and the second surface being modifiable.

Description

[0001] This application is a divisional application of Chinese patent application number 200580014351.7 with an application date of May 6, 2005. technical field [0002] The present invention relates to devices and methods for detecting specific interactions between target and probe molecules. Background technique [0003] Biomedical testing is generally based on the detection of interactions between molecules present in known amounts and locations (molecular probes) and unknown molecules to be detected (molecular target molecules). In modern tests, the probes are arranged in the form of a library of substances on a support, a so-called microarray or chip, so that a sample can be analyzed simultaneously in a parallel fashion with various probes (see e.g. J. Lockhart, E.A. Winzeler, Genomics, gene expression and DNA arrays; Nature 2000, 405, 827-836). The probes referred to herein are usually immobilized on a suitable substrate, such as described in WO 00 / 12575 (see, for exa...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/53B01L3/00B01L7/00G01N21/64G01N33/50
CPCG01N2201/024B01L2300/0816B01L2300/0654G01N21/6428B01L2300/0636G01N21/6452B01L2300/1827B01L2300/0864B01L2300/1838B01L2300/0819B01L7/52B01L2400/0481B01L2300/0877B01L7/525B01L3/502738C12Q1/68G01N21/03B01L3/50851
Inventor 亚力山大·德沃拉克托马斯·艾林格尔尤金·埃曼特劳特托尔斯泰·舒尔茨托马斯·乌尔里希托马斯·凯泽
Owner CLONDIAG GMBH