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Device for controlling the interaction of different kinds of cells, its preparation method and use

A cell and glass substrate technology, used in tissue cell/virus culture devices, biochemical equipment and methods, biochemical cleaning devices, etc., can solve the problems of unstable gold layer properties, high cost, and unfavorable promotion in biological laboratories.

Active Publication Date: 2014-10-01
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In these inventions, the substrates used are all prepared by evaporating a layer of gold on the surface of the glass and using high-vacuum equipment in a clean room. The cost is high, and the property of the gold layer is unstable, so it cannot be stored for a long time, which is not conducive to Biology laboratory promotion

Method used

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  • Device for controlling the interaction of different kinds of cells, its preparation method and use
  • Device for controlling the interaction of different kinds of cells, its preparation method and use
  • Device for controlling the interaction of different kinds of cells, its preparation method and use

Examples

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Effect test

Embodiment 1

[0062] This embodiment is the preparation method of the device capable of adhering, immobilizing and confining three different cells and the method for adhering cells provided by the present invention, which are described in detail as follows. Among them, the schematic diagram of the structure of the device is shown in figure 1 shown.

[0063] 1) Glass slides (Sailboat brand, 60×20 mm), the surface is first soaked in scorpion acid solution (concentrated sulfuric acid: hydrogen peroxide = 3: 1, volume ratio) for 20 minutes, and then washed with a large amount of double distilled water until neutral , dry and ready for use;

[0064] 2) Cut the base of step 1) into small pieces of 2x2 cm, set aside;

[0065] 3) Entrust Beijing Bairen Medical Technology Co., Ltd. to use micromachining technology to prepare a set of concave linear microstructure units on a plexiglass plate. The concave linear microstructure units are composed of nine concave strips; among them, nine concave The...

Embodiment 2

[0073] This example is the preparation method of the device provided by the present invention that can adhere, fix and restrict one kind of cells, and the other two kinds of cells release and diffuse, and the method of adhering the cells, which are described in detail as follows.

[0074] 1) The surface of the glass cover slip was first soaked in a solution of fecivic acid (concentrated sulfuric acid: hydrogen peroxide = 3: 1) for 20 minutes, then washed with a large amount of double distilled water until neutral, dried, and set aside;

[0075] 2) Cut the base of step 1) into small pieces of 2x2 cm, set aside;

[0076] 3) Using micromachining technology, prepare a template comprising four groups of concave linear microstructure units on a plexiglass plate, the concave linear microstructure units of the template are composed of nine concave strips; wherein, the middle of the nine grooves The sections are parallel to each other, and the length of the middle section is 1 cm; the ...

Embodiment 3

[0084] This embodiment is the preparation method of three different cell diffusion devices and the method for adhering cells provided by the present invention, which are described in detail as follows.

[0085] 1) The surface of the glass petri dish is first soaked in a solution of fecivic acid (concentrated sulfuric acid: hydrogen peroxide = 3: 1) for 20 minutes, then washed with a large amount of double distilled water until it is neutral, dried and set aside;

[0086] 2) Using micromachining technology, prepare four groups of concave linear microstructure units on a plexiglass plate, the concave linear microstructure units are composed of nine concave strips; wherein, the middle sections of the nine grooves are parallel to each other, and the middle The segment length is 1.2 centimeters; the second, fifth, and eight grooves on the left side are the first group of equal-section grooves, the width is 300 microns, and the depth of the grooves is 800 microns; the first, third, f...

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Abstract

The invention provides a device for controlling interaction of various cells as well as a manufacturing method and application of the device. The device provided in the invention comprises a glass substrate (1) and a polydimethylsiloxane seal (2) with a plurality of grooves, wherein the polydimethylsiloxane seal is attached to the surface of the glass substrate to respectively form a plurality of micro-channels, the glass substrate in each micro-channel can be selectively modified to be a substrate for promoting cell adhesion by incubating extracellular matrix proteins or can be modified to be a cell adhesion-resistant substrate by assembling polyethylene glycol solution or is not modified. The device is simple and easy to operate, can control fixation, all movement and partial movement of various cells to achieve the purpose of arbitrarily controlling various cells, can be used for researching the interaction among various cells, the pathology or the cell biological development and can also be used for screening medicines.

Description

technical field [0001] The invention relates to a device for controlling the interaction of different kinds of cells, its preparation method and application. Background technique [0002] The current in vitro research system based on the observation and manipulation of only one type of cell has been unable to meet the needs of basic cell biology and pathology research. People often need to observe and manipulate two or more types of cells in vitro under conditions that mimic living organisms. By investigating the interaction between them, we can understand the process of life body development, disease generation and development, and further carry out drug screening and intervention. [0003] The existing devices on the market for studying the interaction between two types of cells, such as the Transwell Chamber, a product of the American company Costar, use a perforated film to separate the two types of cells into upper and lower layers for culture, and can only observe the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/36C12Q3/00C12Q1/02
Inventor 陈振玲陈威肖乐蒋兴宇
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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