Heparin fragment with activity of inhibiting smooth muscle cell proliferation and preparation method thereof

Abstract

The invention discloses a heparin fragment with activity of inhibiting smooth muscle cell proliferation and a preparation method thereof. The preparation method comprises the following steps of: (1) performing enzymatic hydrolysis on heparin by heparinase to obtain enzymatic hydrolysate of heparin; and (2) separating the heparin fragment with the activity of inhibiting smooth muscle cell proliferation from the enzymatic hydrolysate of heparin by an SPR (Surface Plasmon Resonance) sensing technique. The heparin fragment prepared by the method disclosed by the invention has a function of inhibiting the smooth muscle cell proliferation, when the concentration is lower, the inhibition rate of the heparin fragment on the smooth muscle cells is higher than that of heparin, and compared with heparin, the heparin fragment obtained by the invention has very low anticoagulant activity which is only 1.9% of that of the original heparin. Therefore, the method and the prepared heparin fragment disclosed by the invention can be used clinically, and overcome the defect that heparin causes obvious hemorrhage symptom in the use process, so that practical application of the character of heparin of resisting smooth muscle cell proliferation is possible. Thus, the method and product disclosed by the invention have wide application prospects.

Description

technical field [0001] The invention relates to a heparin fragment with the activity of inhibiting the proliferation of smooth muscle cells and a preparation method thereof. Background technique [0002] Heparin is a highly sulfated and important member of the glycosaminoglycan family. After it is synthesized in mast cells, it is transported to various tissues and organs, so it exists widely in organisms. The molecular weight of a heparin single chain in the organism is as high as 60,000-100,000 Da, and many heparin chains are connected by peptides to form a huge proteoglycan molecule. The molecular weight of commercial heparin is derived from the degradation of the above-mentioned glycosaminoglycans, and the giant sugar chains are broken in many places during the production process, forming a product with a molecular weight in the range of about 5000-25000Da. It can be seen that heparin is composed of mixed chains of different lengths, and there are differences between ch...

AI Technical Summary

Problems solved by technology

However, as a highly sulfated polysaccharide, heparin can cause obvious bleeding symptoms during use, and this major side effect limits the clinical application of heparin

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