12 results about "Myocyte proliferation" patented technology

The invention belongs to the technical field of medicines, and discloses an application of a small molecule compound in preparation of a product for preventing and / or treating pulmonary arterial hypertension and complications thereof. The small molecule compound is 8-[[4-(cyclopropanecarbonyl) piperazine-1-yl] methyl]-1, 3-dimethyl-7-(3-methylbutyl) purine-2, 6-diketone, and the structural formula is as shown in a formula (I): # imgabs0. The small molecule compound and pharmaceutically acceptable salts thereof have a remarkable treatment effect on pulmonary arterial hypertension and complications thereof, and can be used for treating pulmonary arterial hypertension and complications thereof. The effects of preventing and / or treating the pulmonary arterial hypertension include the reduction of right heart hypertrophy, the reduction of right ventricular systolic pressure, right heart enlargement, right heart dysfunction, pulmonary vascular remodeling, pulmonary arterial intima thickness, increase of vascular myelization, pulmonary arterial smooth muscle cell proliferation and migration, the reduction of the right ventricular hypertrophy, the reduction of the right ventricular hypertrophy, the reduction of the right ventricular hypertrophy and the reduction of the right ventricular hypertrophy. The pulmonary arterial intima thickness and the vascular myelization level are improved, pulmonary vascular remodeling is reversed, and pulmonary artery smooth muscle cell proliferation and migration are inhibited.

The invention belongs to the technical field of medicines, and discloses application of WZ3146 in preparation of products for preventing and / or treating pulmonary arterial hypertension and complications thereof. The WZ3146 and the pharmaceutically acceptable salt thereof have a remarkable treatment effect on pulmonary arterial hypertension and complications thereof, such as mean pulmonary arterial pressure, high right ventricular systolic pressure, right heart enlargement, right heart dysfunction, pulmonary vascular remodeling, pulmonary arterial intima thickness, increase of vascular myelization, pulmonary artery smooth muscle cell proliferation and migration and the like; the effects of preventing and / or treating pulmonary arterial hypertension comprise expanding the right heart, improving the pulmonary arterial intima thickness and the vascular myelization level, reversing pulmonary vascular remodeling and inhibiting pulmonary artery smooth muscle cell proliferation and migration.

The invention relates to a flexible conductive intravenous stent based on functional electrical stimulation. The invention belongs to the fields of biomedical engineering and the like. The PCL-Gr extravascular stent is prepared by utilizing an electrostatic spinning technology, and the PCL-Gr extravascular stent wraps the periphery of the transplanted vein to provide necessary mechanical support for the transplanted vein so as to maintain the structural stability of the transplanted vein, so that the risk of restenosis caused by hemodynamic change is reduced. And meanwhile, functional electrical stimulation is accurately applied to transplanted veins by utilizing conductivity, so that double mechanisms of promoting endothelialization and inhibiting smooth muscle cell proliferation are achieved, and the risk of intimal hyperplasia is effectively reduced.

The invention discloses a component traditional Chinese medicine preparation for treating moderate and severe uterine prolapse, which is prepared from the following components in percentage by mass: 2.5 to 3.5 percent of rhizoma bletillae gum, 1.0 to 2.0 percent of centella asiatica total glycosides, 0.3 to 0.6 percent of fructus cnidii oil, 0.2 to 0.5 percent of cinnamon oil, 0.001 to 0.005 percent of NP-700 type sodium polyacrylate, 0.35 to 0.65 percent of B type carbomer, 4.5 to 7.5 percent of glycerol, 0.35 to 0.65 percent of triethanolamine, 0.05 to 0.09 percent of chlorhexidine acetate and the balance of purified water. The sum of the mass percentages of the components is 100%. The specific preparation method comprises the following steps: weighing the components, dissolving, mixing, adjusting the pH value, and forming. The traditional Chinese medicine preparation is used for preparing medicines for treating moderate and severe uterine prolapse, has the effects of warming yang and promoting tissue regeneration, and can repair degenerated and damaged pelvic floor muscle groups by promoting collagen expression, extracellular matrix generation and muscle cell proliferation in pelvic floor tissues after vaginal administration; the structural strength and supporting tension of pelvic floor tissue are improved, the supporting and lifting effect on the uterus is recovered, and the prolapsed uterus is stably reset.

The invention relates to application of an FGFBP2 gene in regulation and control of growth and development and cell cycle of chicken skeletal muscle. The inventor finds the existence of the FGFBP2 gene in early-stage work of a team, and also finds that the expression quantity difference of the gene in muscular tissues of chickens in different development stages and varieties is obvious. In order to further reveal the effect of the FGFBP2 gene in the growth and development process of chicken skeletal muscles, the FGFBP2 gene is overexpressed in chicken myoblasts and AA broiler chickens respectively by using an overexpression technology, and detection finds that the overexpressed FGFBP2 gene can promote the proliferation of myoblasts. The discovery that the FGFBP2 gene can regulate and control the proliferation function of the chicken skeletal muscle cells provides a new theoretical basis for revealing the development of the chicken skeletal muscle and improving the meat production character, and provides a new thought for improving the meat production performance of livestock.

The invention relates to a myogenic cell rapid proliferation gene knock-in bovine primary cell and a preparation method thereof, and belongs to the technical field of gene editing and biology. Specific sgRNA is designed aiming at a bovine genome safety site H11, and directional insertion of a target gene is realized by taking a gene containing a homologous arm and a muscle-promoting cell proliferation gene Ufc1 and a gene Ufl1 as a donor template through Cas9 protein mediated DNA double-strand cutting. The method has the advantages that the cutting efficiency of the safety site H11 is improved to 90% or above, so that the obtaining rate of the positive monoclonal cell strain can be remarkably improved; the ssDNA is used as a donor fragment to be introduced into bovine fibroblasts, the survival rate of electrotransfected cells is two times that of double-stranded plasmid DNA, and meanwhile, the transfection efficiency is improved by more than four times. The cell proliferation efficiency of the Ufc1 gene knock-in cell strain is improved by 1.6 times compared with that of a wild type, and the Ufc1 gene knock-in cell strain is applied to cattle and provides important technical support for genetic improvement of muscle tissue engineering and animal husbandry.

The application discloses a use and application based on CDKN1A influence on proliferation and differentiation of goat skeletal muscle, relates to the field of agricultural genetic engineering, and discloses that iRNA-seq and RNA-seq technology reveals mRNA expression maps of the longest dorsal muscle of 5-month-old Hainan black goats and 24-month-old Hainan black goats in two development stages, and CDKN1A with differential expression is screened out; the CDKN1A gene is used for negatively regulating proliferation of goat myoblasts; and the application finds that CDKN1A has a regulation effect on proliferation and differentiation of skeletal muscle cells of Hainan black goats, and can be applied to development and utilization of germplasm resources of the breed and seed selection and breeding.

This invention relates to the field of biomimetic nanozymes and their preparation technology, disclosing a biomimetic nanozyme, its preparation method, and its applications. A peptide-functionalized biomimetic nanozyme (HRPL) is obtained by loading rapamycin onto HMPB nanozymes, coating the surface with a platelet membrane, and functionalizing it with the integrin-binding peptide LXW7. The binding of the platelet membrane to the LXW7 peptide enables targeted delivery to the site of endothelial injury, promoting endothelial repair and re-epithelialization. In an acidic inflammatory microenvironment, HRPL releases rapamycin, inhibiting smooth muscle cell proliferation and migration, and preventing restenosis. HRPL also scavenges reactive oxygen species, reducing oxidative stress and local inflammation, thereby improving the vascular microenvironment. The therapeutic effect was evaluated using a typical rat carotid balloon injury model. This dual-targeting mechanism not only promotes endothelial repair but also reduces restenosis and inflammation, showing significant clinical therapeutic potential. Especially after stent implantation, HRPL helps improve prognosis, reduce complications, and restore vascular homeostasis.

The application belongs to the technical field of medicine, and discloses a kind of oxaevodiamine compounds and pharmaceutical use thereof, and specifically provides a kind of oxaevodiamine compounds shown in formula (I), and their physiologically acceptable salts and pharmaceutical compositions.The compounds can effectively inhibit the proliferation of pulmonary artery smooth muscle cells caused by PDGF-BB, and can be used for preventing or treating pulmonary arterial hypertension.

An implantable device including a matrix and a zinc-containing layer at least partially covering the substrate. The zinc-containing layer includes a zinc compound. The ratio of the mass of the zinc element in the zinc-containing layer to the surface area of the implantable device is 0.1-200 μg / mm2. The average porosity of the zinc-containing layer is 30% or less. By matching the ratio of the mass of zinc in the zinc-containing layer to the surface area of the implantable device with the average porosity of the zinc-containing layer, the release rate of zinc is controlled within a reasonable range so that the rate of formation of the zinc-containing substance is controlled such that the concentration of the zinc-containing substance accumulated in the tissue is higher than the concentration which inhibits the proliferation of smooth muscle cells, and is always lower than the toxic concentration which causes cell death.

An antisense oligonucleotide of 15-30 bases or a salt or a solvate thereof, wherein the antisense oligonucleotide has a nucleotide sequence complementary to a target site in exon 1 of the myostatin gene and is capable of inhibiting the production of the mRNA of the myostatin gene. A pharmaceutical drug, a food, a feed, an agent for promoting myocyte proliferation and / or hypertrophy, an agent for increasing muscle mass and / or inhibiting muscle weakness, an agent for inhibiting production of the mRNA of the myostatin gene, and an inhibitor of the function of myostatin, each of which comprises the above antisense oligonucleotide or a salt or a solvate thereof.

The application discloses a bovine muscle development related enhancer Enh483 and application thereof in promoting bovine myoblast proliferation and / or differentiation. The application predicts and annotates the enhancer by IGV visual analysis of chromatin open regions by using existing buffalo myoblast proliferation period and differentiation period ATAC-Seq data and Chip-seq data, screens out a candidate enhancer Enh483 related to muscle development, and detects the enhancer activity by a dual luciferase vector report system. After the Enh483 activity is inhibited, the buffalo myoblast proliferation and differentiation are inhibited, which indicates that the Enh483 can promote the buffalo myoblast proliferation and / or differentiation. The application provides an important theoretical basis for the mechanism of the transcriptional regulatory elements such as enhancers in muscle development, and provides data support for buffalo breeding and strain selection.