The invention discloses a Zn-Mg1Ca series zinc alloy and preparing method and application of Zn-Mg1Ca series zinc alloy. The zinc alloy comprises Zn and Mg1Ca, wherein the mass percent of Mg1Ca in the zinc alloy ranges from 0 to 10% but not includes 0. The zinc alloy further comrpsies a microelements which are at least one of silicon, phosphorus, lithium, silver, tin and rare earth elements. The mass percent of the microelements ranges from 0 to 3% but not includes 0. The mechanical property of the Zn-Mg1Ca series zinc alloy meets the requirement for strength and toughness of medical implant materials, the Zn-Mg1Ca series zinc alloy is free of cytotoxicity on endothelial cells and bone-forming cells, can inhibit smooth muscle cell proliferation and has good tissue compatibility and blood compatibility, meanwhile, the Zn-Mg1Ca series zinc alloy can also be regulated to be degraded by body liquid, metal ions which are dissolved out can be absorbed and utilized by an organism or metabolized to be discharged out of the body, and the Zn-Mg1Ca series zinc alloy has good antibacterial performance and can be applied to preparation of medical implants.