Simple culture method for effective microorganisms (EM)

A culture method and technology for EM bacteria are applied in the field of culture of mixed bacteria to achieve the effects of convenient handling, small impact on production environment and reduced investment cost

Active Publication Date: 2011-08-17
DONGGUAN SHENGYUAN ENVIRONMENTAL PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The demand for the development of EM bacteria has a very wide application prospect, but the existing methods for producing EM bacteria need to be improved in terms of production cost and production process. Urgent needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1.1 Configure culture medium

[0021] Sterilize and disinfect 20L plastic square barrels and measuring cylinders with ventilated lids.

[0022] Weigh 6% molasses water and 0.5% peptone respectively by weight, add purified water, and configure a total of 20 kg of culture solution.

[0023] 1.2 Inoculation of Bacillus

[0024] Weigh 1 kg of bacillus (purchased from Changan Biological Co., Ltd.), and put the weighed bacteria into the culture solution.

[0025] 1.3 Incubate the inoculated transparent plastic bucket at 20-35°C for 24 hours.

[0026] 1.4 Inoculation of compound lactic acid bacteria, yeast and photosynthetic bacteria

[0027] Weigh 1.2kg of compound lactic acid bacteria (purchased from Guangming Dairy Factory), 1.0kg of saccharomyces (purchased from Angel Yeast Co., Ltd.), and 0.8kg of photosynthetic bacteria (purchased from Changan Biological Co., Ltd.), and put the weighed strains into into the culture medium.

[0028] 1.5 Cultivate the inoculated trans...

Embodiment 2

[0030] 2.1 Configure culture medium

[0031] The 25L plastic square barrels and measuring cylinders with ventilated lids were sterilized.

[0032] Weigh 6% molasses water and 0.5% peptone respectively by weight, add purified water, and configure a total of 25 kg of culture solution.

[0033] 2.2 Inoculation of Bacillus

[0034] Weigh 1.25 kg of bacillus (purchased from Changan Biological Co., Ltd.), and put the weighed strains into the culture solution.

[0035] 2.3 Incubate the inoculated transparent plastic bucket at 25-35°C for 24 hours.

[0036] 2.4 Inoculation of compound lactic acid bacteria, yeast and photosynthetic bacteria

[0037] Weigh 1.5kg of compound lactic acid bacteria (purchased from Guangming Dairy Factory), 1.25kg of saccharomyces (purchased from Angel Yeast Co., Ltd.), and 1.0kg of photosynthetic bacteria (purchased from Changan Biological Co., Ltd.), and put the weighed strains into into the culture medium.

[0038] 2.5 Cultivate the inoculated transp...

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PUM

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Abstract

The invention relates to a culture method of mixed bacteria, in particular to a culture method for EM. The simple culture method for EM comprises the following steps: A, preparing culture solution, and filling into a plastic square barrel with a ventilating cover; B, inoculating bacillus; C, culturing under a closed condition for 24 hours; D, inoculating composite lactic acid bacteria, saccharomycetes and photosynthetic bacteria; and E, culturing under a closed condition. In the invention, a cheap plastic square barrel which is common on market is used as an EM culture container; generally, culture can be performed at normal temperature; culture and use on site can be realize; and the method has the advantages of environment protection, flexible production operation, production and use cost conservation and the like.

Description

technical field [0001] The invention relates to a method for cultivating mixed bacteria, in particular to a method for cultivating EM bacteria. Background technique [0002] EM bacteria, its English name is "Effective Miero-origanisms", which is a live bacterial preparation made of more than 80 kinds of microorganisms from more than 10 genera such as photosynthetic bacteria, actinomycetes, yeasts, compound lactic acid bacteria, and Bacillus. , Various microorganisms in the EM microbial agent symbiosis, interact and develop together, and play a variety of functions, which can quickly promote the decomposition of organic substances such as animal and plant residues, and inhibit the growth of spoilage bacteria and other germs; in the water ecological environment, EM bacterial agent can effectively inhibit the growth of spoilage organisms and some pathogenic bacteria, make it difficult for odorous substances such as ammonia and sulfide to form, and accelerate the decomposition o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/16C12N1/20C12R1/01C12R1/07C12R1/225C12R1/645
Inventor 熊幼林
Owner DONGGUAN SHENGYUAN ENVIRONMENTAL PROTECTION TECH
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