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Human osteosarcoma cell strain and application thereof

A technology of human osteosarcoma and cells, which is applied in the direction of tumor/cancer cells, animal cells, vertebrate cells, etc., can solve the problems of low biodiversity and large differences in biological properties of osteosarcoma, and achieve high metastatic potential and stable properties Effect

Inactive Publication Date: 2013-11-06
姚阳 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved in the present invention is to provide a new human osteosarcoma cell line and its purpose

Method used

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  • Human osteosarcoma cell strain and application thereof
  • Human osteosarcoma cell strain and application thereof
  • Human osteosarcoma cell strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Establishment of primary osteosarcoma cell line SF-86 and identification of biological characteristics

[0063] 1. Experimental materials:

[0064] 1.1 The source of primary cell tissue and the main reagents required for cell culture:

[0065] Tissue source: A 17-year-old male patient. In May 2008, he underwent high-position amputation of the left thigh due to osteosarcoma of the left upper femur. The pathological diagnosis of osteosarcoma (chondroblastic type) was performed. Adjuvant chemotherapy, a mass on the right upper arm and left hip was found in June 2008, and a mass on the right upper arm and left hip was removed in June 2008. Postoperative pathology: metastatic osteosarcoma, immunohistochemistry: p53(+), Vim( +), Des(-), SMA(-), Ki67(+), CD117(-), CD99(+), Bcl-2(+), MG(-), multiple occurrences in both lungs were found in July 2008 Metastases, and in August 2008, another subscalp soft tissue metastasis was found on the right side. In this experiment, the ...

Embodiment 2

[0152] Example 2 Establishment of a human osteosarcoma high metastasis model using primary osteosarcoma cell lines

[0153] 1. Experimental materials:

[0154] Twenty BALB / c-nu / nu nude mice were provided by the Experimental Animal Center of Shanghai Sixth People's Hospital. The mice were 3 to 4 weeks old and male, weighing 15-18 g.

[0155] Experiments and breeding were carried out in an ultra-clean laminar flow rack under SPF-level conditions. Sterilized water and SPF-level special feed were provided for free intake by animals. The above experimental conditions were provided by the Experimental Animal Center of Shanghai Sixth People’s Hospital.

[0156] See the first part for the remaining experimental materials and reagents.

[0157] The antibodies used in the immunohistochemistry of transplanted tumors were provided by the Department of Pathology, Shanghai Sixth People's Hospital.

[0158] 2. Experimental method:

[0159] 2.1 Cell culture conditions:

[0160] SF-86 cell line in regular ...

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Abstract

The invention provides a human osteosarcoma cell strain and application thereof. The human osteosarcoma cell strain is collected in the China Center for Type Culture Collection with the collection number of CCTCC NO:C201002. The human osteosarcoma cell strain can be used for producing human osteosarcoma in a mammal to prepare a human osteosarcoma animal model, and can be further used for screening a drug candidate for treating human osteosarcoma. The human osteosarcoma cell strain provided by the invention has stable property, and can be stably subjected to passage repeatedly. The property of the human osteosarcoma cell strain keeps stable from the 30th generation to the 90th generation during in-vitro passage. The human osteosarcoma cell strain has the clinical biological property of human osteosarcoma, and particularly has the characteristics of primary drug resistance and high transfer potential. The human osteosarcoma cell strain plays an important role in solving the bottleneck problems of primary drug resistance and remote transfer which cannot be solved in the conventional human osteosarcoma treatment, and is an ideal cell strain for the basic research of human osteosarcoma and the preclinical phase application.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to a human osteosarcoma cell line and its application. Background technique [0002] As early as 5000 years ago on ancient Egyptian mummies, medical researchers discovered the existence of osteosarcoma (Marina N, etal. Biology and therapeutic advances for pediatric osteosarcoma. Oncologist 2004; 9(4): 422-41). Until now, osteosarcoma is the most common primary malignant bone tumor in children and adolescents. Especially before the age of 20, patients with osteosarcoma account for nearly 60% of all malignant bone tumor patients (Saeter G, Kloke O, JelicS, et al. ESMO Minimum Clinical Recommendations for diagnosis, treatment and follow-up of osteosarcoma. Ann Oncol. 2005 ; 16Suppl 1: i71-2), the disease can occur at any age, but 75% of osteosarcoma patients are between 10 and 25 years old. There are slightly more males than females, and the ratio is about 1.5:1. Generally, 80% to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09A01K67/027A61K49/00C12R1/91
Inventor 姚阳沈赞余文熙
Owner 姚阳
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