Preparation method of inactivated vaccine for iridovirus of grouper
A technology of iridescent virus and inactivated vaccine, which is applied in antiviral agents, virus antigen components, etc., can solve the problem of not seeing grouper iridescent virus inactivated vaccine, etc., and achieve good immune protection effect, simple equipment requirements, and inactivated vaccines. The effect of short life time
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0014] 1. Cultivation of grouper embryonic cells for amplification of grouper iridescent virus. Take grouper embryo cells covered with a single layer, remove the original culture medium, rinse with phosphate buffered saline solution, add 0.25% trypsin to digest for 2 minutes, add the newly prepared MEM culture medium containing 10% fetal bovine serum (pH7 .4), after blowing evenly, divide it into 3 cell culture flasks for culture and expansion at 25°C, and inoculate grouper iridescent virus amplification when the cells grow to the logarithmic phase.
[0015] 2. Amplification and harvest of grouper iridescent virus. Inoculate 0.05 MOI (multiplicity of infection, multiplicity of infection) virus in logarithmic phase cells and culture in a 25°C incubator to amplify the virus. After 4 days of continuous culture, the cytopathy was complete. After repeated freezing and thawing 3 times, divide into 50 ml centrifuge tubes, centrifuge at 2500g for 10 minutes to remove cell debris, col...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com