Goat TNNT3 (human fast skeletal troponin T) gene and method for cloning complete sequence of gene coding region of goat TNNT3 gene
A cloning method and coding region technology, applied in the field of goat TNNT3 gene and cloning the complete sequence of goat TNNT3 gene coding region, can solve the problem of no goat TNNT3 gene
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[0021] The present invention will be described in detail below in conjunction with specific embodiments.
[0022] Step 1 extracts the total RNA (ribonucleic acid) in the goat leg muscle tissue, and then reverse-transcribes the total RNA into cDNA;
[0023] 1. Collection of Tissue
[0024] Immediately after bleeding from the carotid artery of one-year-old Tianfu mutton sheep, about 1 g of leg muscle was taken and placed in a liquid nitrogen tank for total RNA extraction
[0025] 2. RNA preparation and cDNA synthesis
[0026] Grind the goat leg muscle into powder with liquid nitrogen in the laboratory, put it into a 1.5ml EP tube, and put it in a minus 80 ultra-low temperature refrigerator for later use.
[0027] Take a new 1.5ml EP tube, add 1.2ml of Trizol solution, and then add about 80mg of leg muscle tissue powder. Immediately shake and mix by hand, shake with a shaker for about 30 seconds, and then place at room temperature for 4 minutes.
[0028] Centrifuge at 13500rp...
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