Extraction method of genome DNA of isolated soy proteins
A soybean protein isolate and extraction method technology, applied in the field of genomic DNA extraction, can solve problems such as inability to extract genomic DNA, and achieve the effects of facilitating rapid detection, simple operation, and short time-consuming
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[0006] The preferred embodiment of the present invention is as follows: firstly, the soybean protein isolate and TE buffer solution are fully mixed to form a TE mixed solution, and then the guanidine isothiocyanate lysate with twice the volume of the mixed solution is added, fully mixed, and lysed at room temperature. Add an equal volume of phenol, chloroform / isoamyl alcohol to extract protein, add an equal volume of chloroform / isoamyl alcohol to the supernatant after centrifugation to extract protein, add an equal volume of chloroform to the supernatant to extract protein after centrifugation, and centrifuge , the supernatant was precipitated with isopropanol, centrifuged, the precipitate was washed with ethanol, dried at room temperature, and dissolved in TE buffer to obtain a TE solution of genomic DNA.
[0007] The soybean protein isolate in the TE mixed solution is 30-80 mg, and the TE buffer solution is 120-320 μL. The preferred range is 50 mg of soybean protein isolate ...
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