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Nucleotide sequences, methods and kits for detecting hpv

A technology for detection kits and sequences, used in biochemical equipment and methods, determination/inspection of microorganisms, etc.

Active Publication Date: 2011-10-05
DIAGCOR LIFE SCI LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, HCII can only detect 18 HPV subtypes (13 HCII high-risk types, 5 HCII low-risk types), which is much lower than the 38 most common human HPV subtypes

Method used

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  • Nucleotide sequences, methods and kits for detecting hpv
  • Nucleotide sequences, methods and kits for detecting hpv
  • Nucleotide sequences, methods and kits for detecting hpv

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Embodiment Construction

[0026] The invention will now be described in the following paragraphs by way of example and with reference to the accompanying drawings. The objects, features and aspects of the present invention are disclosed or apparent through the following description. Those of ordinary skill in the art will appreciate that the discussions herein are a description of exemplary embodiments only, and are not intended to limit the broader aspects of the invention embodied in exemplary illustrations.

[0027] The inventors of the present invention have prepared probes for various HPV subtypes, the nucleotide sequences of which are complementary to HPV DNA, so that a relatively small number of probes can detect most common HPV subtypes. In a specific embodiment, at least 36 of the most common HPV subtypes can be detected by various combinations of 2 to 4 HPV probes of the present invention. The probe of the present invention contains the nucleotide sequences shown in SEQ ID NOs. 1-24 (Table 1...

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Abstract

Various sequences as detection probes for human papillomavirus (HPV), in which each of these sequences is able to detect multiple subtypes of HPV, are provided. HPV detection chips, detection kits comprising these probes and HPV detection method by using these probes are also provided. With the sequences of the current invention, it is possible to detect commonly seen 38 subtypes of HPV, including those of high risk, with various combinations of the sequences of the current invention.

Description

technical field [0001] The present invention relates to nucleotide sequences, methods and kits for the detection of human papillomaviruses (HPV), especially those that use a relatively small number of nucleotide sequences as probes to detect multiple HPV subtypes. Background technique [0002] Cervical cancer is one of the most common cancers in women. The worldwide death rate was estimated at 273,500 in 2002 and increased to 320,000 in 2004. An estimated 83% of these patients are in developing countries, accounting for 15-24% of cancers in women, much higher than the 3-7% in developed countries. Cervical cancer has been found to be closely associated with human papillomavirus (HPV) infection, which can lead to cervical intraepithelial neoplasia (CIN), a precancerous stage during cancer development. Therefore, detection of HPV plays an extremely important role in the prevention and treatment of cervical cancer. [0003] Methods for detecting HPV in humans using nucleotide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/708C12Q2600/156
Inventor 谭荣安周国辉陈卓宇
Owner DIAGCOR LIFE SCI LTD
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