Monoclonal antibody capable of specifically identifying HPV18 L1 protein and application of monoclonal antibody
A monoclonal antibody and specific technology, applied in the field of HPV infection detection, can solve the problems of expensive equipment, inability to judge recovery and prognosis, and not suitable for large-scale screening of cervical cancer, and achieve high specificity and convenient detection
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[0031] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention.
[0032] 1. Immunogen design and monoclonal antibody preparation
[0033] We synthesized a modified polypeptide (L1M186) containing the specific epitope QSPVP from HPV18 L1 protein; and L1M186-coupled hemocyanin (KLH) constituted L1M186-KLH as an immunogen.
[0034]The immunogen was dissolved in phosphate buffered saline (PBS, pH7.4) at a concentration of 1 mg / ml. The primary immunization was performed at week 0, and each mouse was fully emulsified with 100 μl of 1 mg / ml immunogen solution and 100 μl of complete Freund’s adjuvant to form a water-in-oil emulsion, and immunized by multi-point abdominal subcutaneous injection. 6 mice. In the 2nd week, 5th week, 8th week, and 11th week, the booster immunization was carried out 4 times, and each time each...
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