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Integrated utilization technology of mulberry leaves

A technology of mulberry leaves and technology, which is applied in the field of comprehensive utilization of mulberry leaves, can solve problems such as mulberry leaf extraction, achieve the effects of increasing added value, saving food resources, and solving environmental pollution problems

Active Publication Date: 2013-02-13
ZHEJIANG QIANCAOSU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] One of the purposes of the present invention is to provide a process for the comprehensive utilization of mulberry leaves, which combines fermentation and extraction to effectively solve the problem of residues after mulberry leaf extraction

Method used

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  • Integrated utilization technology of mulberry leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Liquid culture of Monasucs anka:

[0029] Liquid medium composition (weight percent): glucose 2%, sucrose 2%, rice flour 1%, soybean flour 1%, MgSO 4 0.1%, ZnSO 4 0.1%, KH 2 PO 4 0.2%, the balance is distilled water, and the pH value is natural. 500mL capacity is 250mL, and then the liquid culture is sterilized at 121°C for 20 minutes. After cooling to 40°C, inoculate Monascus spores greater than 10 8 15mL of the spore suspension of Monascus spores / mL, and cultured at 32°C and 180r / min for 72h as liquid Monascus species.

[0030] Weigh 500 grams of dried mulberry leaves, add 8 times of water, extract at 100°C for 1 hour, filter, add 6 times of water to the obtained filter residue, extract at 100°C for 30 minutes, filter, combine the filtrates, concentrate to a specific gravity of 1.15g / mL , spray-dried to obtain mulberry leaf extract, the mulberry leaf crude polysaccharide in the obtained mulberry leaf aqueous extract reached 111 mg / g, and the GABA content reach...

Embodiment 2

[0033] Liquid culture of Monasucs purpureus:

[0034] The composition of the liquid medium (weight and volume percentage): glucose 2%, sucrose 2%, rice flour 1%, soybean flour 1%, MgSO 4 0.1%, ZnSO 4 0.1%, KH 2 PO 4 0.2%, the balance is distilled water, and the pH value is natural. The volume of the 500mL triangular flask is 250mL, and then the liquid culture is sterilized at 121°C for 20 minutes. After cooling to 40°C, inoculate the purple red yeast rice spores eluted from the slope for more than 10 8 10mL of the spore suspension of Monascus spores / mL, and cultured at 32°C and 180r / min for 72h as liquid Monascus species.

[0035] Weigh 500 grams of dried mulberry leaves, add 8 times of water, extract at 80°C for 2 hours, filter, add 6 times of water to the obtained filter residue, extract at 100°C for 1 hour, filter, combine the filtrates, concentrate to a specific gravity of 1.1g / mL , and spray-dried to obtain mulberry leaf extract. The obtained mulberry leaf extract...

Embodiment 3

[0038] Liquid culture of Monasucs ruber:

[0039] Liquid medium composition (weight percent): glucose 2%, rice flour 1%, soybean flour 1%, MgSO 4 0.1%, ZnSO 4 0.1%, KH 2 PO 4 0.2%, the balance is distilled water, and the pH value is natural. 500mL capacity is 250mL, and then the liquid culture is sterilized at 121°C for 20 minutes. After cooling to 40°C, inoculate with more than 10 red yeast spores eluted from the slant. 8 10 mL of Monascus spore suspension per mL was cultured at 32°C and 180 r / min for 72 hours as a liquid Monascus species.

[0040] Weigh 500 grams of dried mulberry leaves, add 10 times of water, extract at 100°C for 2 hours, filter, add 6 times of water to the obtained filter residue, extract at 100°C for 1 hour, filter, combine the filtrates, concentrate to a specific gravity of 1.1g / mL , carry out spray drying, obtain mulberry leaf extract, gained mulberry leaf extract is tested, and mulberry leaf crude polysaccharide is 102mg / g, and GABA is 17.35mg / ...

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Abstract

The invention belongs to the field of mulberry leaf processing, and specifically relates to a process for comprehensive utilization of mulberry leaves, comprising the following steps: (1) adding 6-10 times the weight of water to the dried mulberry leaves, extracting at 80-100°C, and extracting The mulberry leaves are filtered to obtain the mulberry leaf residue and mulberry leaf extract. (2) The mulberry leaf extract is concentrated and then spray-dried to obtain the mulberry leaf extract. (3) The mulberry leaf residue is added with carbon source and nitrogen source , adjust the water content to 30-45% to obtain the compost, inoculate the Monascus species after the compost is sterilized, and then ferment, and the fermented product is dried and pulverized to obtain the mulberry leaf red yeast rice. The process of the present invention combines extraction and fermentation, on the one hand, it solves the environmental problems caused by the slag after leaf extraction; The unextracted active ingredients in mulberry leaves can be brought into the following mulberry leaf red yeast rice, so as to strengthen the function of red yeast rice.

Description

technical field [0001] The invention belongs to the field of mulberry leaf processing, and in particular relates to a process for comprehensive utilization of mulberry leaves. Background technique [0002] Mulberry leaves, also known as fairy leaves, have the effects of nourishing yin and blood, dispelling wind and heat dissipation, benefiting liver and ventilation, reducing blood pressure and diuresis, and are both food and medicine raw materials announced by the Ministry of Health. [0003] At present, a variety of active ingredients have been isolated from mulberry leaves and their efficacy has been proved: such as mulberry leaf polysaccharides (Xing Dongxu et al., Comparison of medicinal effects of hot water extraction and microwave extraction of mulberry leaf polysaccharides and a preliminary study on the mechanism of hypoglycemia, Sericulture Science, 2008 Issue 03), glycosidase inhibitor 1-deoxynojirimycin (DNJ), gamma-aminobutyric acid (GABA) with the function of reg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02A23L1/29A61K36/605A23L33/00
Inventor 陈小林
Owner ZHEJIANG QIANCAOSU BIOTECH CO LTD
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