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Total saponins of psammosilene tunicoids (TSPT) with antitumor activity, and preparation thereof

A technology with anti-tumor activity and total saponins, which can be used in anti-tumor drugs, medical preparations containing active ingredients, plant raw materials, etc.

Inactive Publication Date: 2011-11-09
王学勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Before the present invention, there were no reports on the anti-tumor effect and clinical application of Jintiesuo

Method used

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  • Total saponins of psammosilene tunicoids (TSPT) with antitumor activity, and preparation thereof
  • Total saponins of psammosilene tunicoids (TSPT) with antitumor activity, and preparation thereof
  • Total saponins of psammosilene tunicoids (TSPT) with antitumor activity, and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The preparation method of the total saponins of embodiment 1 gold iron lock

[0016] The optimized extraction conditions are as follows: take 3 kg of Jintiesuo medicinal material, reflux extraction with 8 times the volume of 95% ethanol at 76°C for 3 times, each time for 1 hour, combine the extracts, recover the ethanol under reduced pressure until dry, and then extract Jintiesuo The substance was dissolved with low-carbon alcohol, passed through a macroporous adsorption resin column, and eluted with 10%, 30%, 50%, 70%, and 90% ethanol-water solution as the mobile phase, collected the eluate, and concentrated under reduced pressure to remove the solvent , freeze or spray-dry to powder to obtain 151.3g of total saponins of Jintielock.

Embodiment 2

[0017] Example 2 Inhibitory effect of total saponins of aureosides on 5 kinds of tumor cells in vitro

[0018] Cells: breast cancer cells (MCF-7), human colon cancer cells C26, human liver cancer cells BEL-7401, human ovarian cancer cells (SKOV3), human gastric cancer cells (BGC-823), the above 5 tumor cells were provided by Chinese Medicine Provided by the Cancer Hospital of the Academy of Sciences.

[0019] Drugs and reagents: total saponins of Jintielock, provided by the Molecular Pharmacognosy Laboratory of Beijing University of Traditional Chinese Medicine, white powder, penicillin (Sigma, USA), streptomycin (Sigma, USA); trypsin (Sigma, USA), RPMI1640 medium ( Gibco, Invitragen co., USA); fetal bovine serum (Gibco, Invitragen co., USA); dimethyl sulfoxide (DMSO) (Sigma, USA), tetramethylazolium blue (MTT) (Sigma, USA ). Culture conditions: temperature 37°C, humidity 100%, culture in air containing 5% carbon dioxide.

[0020] Instruments: CO2 incubator (Thermo 311); ul...

Embodiment 3

[0025] Example 3 The effect of total saponins of aureoside on normal splenocytes

[0026] Spleen Cell Immune Cell Culture

[0027] Six- to eight-week-old Kunming mice were sacrificed by cervical dislocation, soaked in 75% ethanol for 3 minutes, took out the mice and placed them on sterile paper with the left ventral side up; cut a small incision in the middle of the left ventral side of the mice, and tore off the skin The abdominal wall was exposed, and the red strip-shaped spleen was visible; the peritoneum was lifted on the underside of the spleen, cut open and turned upside down to expose the spleen, the spleen was lifted with tweezers, and the connective tissue under the spleen was separated with ophthalmic scissors, and the spleen was taken out. Put it into a petri dish filled with 5mL of Hanks' solution; place the spleen on a stainless steel mesh (200 mesh), press the spleen gently with a needle core of a syringe to obtain a cell suspension. Culture medium: RPMI 1640 wi...

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Abstract

The invention discloses a new application of the antitumor effect of a Miao ethnomedicine psammosilene tunicoids, and simultaneously discloses a preparation method and preparation of the pharmaceutical part of psammosilene tunicoids with the antitumor activity. The preparation method mainly comprises the steps of: extracting by ethanol, concentrating, extracting by n-butyl alcohol, purifying by macroporous absorption resin, and the like to obtain the total saponins of psammosilene tunicoids (TSPT). The total saponins are used as raw materials to prepare clinical commonly used pharmaceutical preparations such as tablets, capsules, injections and the like. A method of pharmacodynamic evaluation both in vivo and in vitro is adopted, wherein shown by in vitro tests, the TSPT has stronger inhibitory actions on 6 tumor cells of stomach cancer, liver cancer, colon cancer, ovarian cancer, breast cancer and melanoma, but has unobvious inhibition on the normal splenic lymphocyte; shown by integral animal in vivo experimental results, the TSPT has an inhibition rate as high as 72% on S180 solid tumors and has high action valence, but has small influence on the body weight of mice; and compared with a positive drug cis-platinum, the TSPT has lower side effect. Shown by the experimental results, the TSPT has strong anticancer valence, low side effect and the advantage of original innovation.

Description

technical field [0001] The invention relates to the application of the effective part of Jintiesuo-total saponins in antitumor effect and its preparation. Background technique [0002] Malignant tumors have always been one of the major diseases that seriously threaten human health. Drug toxicity and adverse reactions of anticancer drugs have also been paid more and more attention. Researchers from all over the world have been making unremitting efforts to find candidate drugs with certain selectivity against tumors. It is the current and future research focus and focus to seek drugs with less toxic side effects, strong anti-tumor effect and certain selectivity. Natural medicines are a treasure trove of ideal antitumor drug candidates. A number of natural medicines with strong antitumor activity, such as paclitaxel and camptothecin, have been screened out, showing good clinical efficacy and application prospects. Therefore, in view of the rich resources of natural herbal m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/36A61P35/00
Inventor 王学勇赵保胜刘春生高伟张园园王秀丽张元
Owner 王学勇
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