Method for extracting bio-oil by recycling botryococcus braunii cells

A technology of Botrytis brannica and biomass oil, which is applied in the field of reusing Botrytis brannicola cells to extract biomass oil, which can solve the problems of strong light, slow growth, and low culture density, so as to improve the production intensity of oil and reduce the processing cost , the effect of rapid oil accumulation

Inactive Publication Date: 2013-03-27
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Description
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Problems solved by technology

However, in the process of large-scale cultivation of microalgae to produce biofuels, there

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Collect the wet algae with a certain volume of Botrytis brachyphyllum culture solution with a biomass of 2 g / L or more after filtering, the water content of the wet algae is 90%; put the wet algae into a stirring reactor, add n-hexane at room temperature An extraction solvent composed of alkane and ethylene glycol dimethyl ether (100:2, v / v) was used to extract the biomass oil of Botrytis brachyphytum, and the amount of extraction solvent was 6 times the volume of the wet algae, and stirred at a speed of 400rpm for 10min , separate the algae cells and collect the extraction solvent containing oil, add new extraction solvent again, repeat the extraction operation steps, the wet algae body is extracted 3 times in total, and the total oil extraction rate is 30%; Solvents are combined, vacuum rotary evaporation is carried out, the extraction solvent is recovered for reuse, and the obtained biomass oil is collected; the wet algae cells extracted from the biomass oil are added...

Embodiment 2

[0030]Collect the wet algae with a certain volume of Botrytis brachyphylla culture solution with a biomass of 2 g / L or more after filtering, the water content of the wet algae is 95%; put the wet algae into a stirring reactor, add the An extraction solvent composed of alkane and ethylene glycol dimethyl ether (100:1, v / v) was used to extract the biomass oil of Botrytis brachyphytum, and the amount of extraction solvent was 4 times the volume of the wet algae body, and stirred at a speed of 200rpm for 15min , separate the algae cells and collect the extraction solvent containing oil, add new extraction solvent again, repeat the extraction operation steps, the wet algae body is extracted twice in total, and the total oil extraction rate is 45%; Solvents are combined, vacuum rotary evaporation is carried out, the extraction solvent is recovered for reuse, and the obtained biomass oil is collected; the wet algae cells extracted from the biomass oil are added to a fresh medium 25 ti...

Embodiment 3

[0032] Collect the wet algae with a certain volume of Botrytis brachyphyllum culture solution with a biomass of 2 g / L or more after filtering, the water content of the wet algae is 92%; put the wet algae into a stirring reactor, add the An extraction solvent composed of alkanes and ethylene glycol dimethyl ether (100: 1.5, v / v) was used to extract the biomass oil of Botrytis brachyphytum, and the amount of extraction solvent was 5 times the volume of the wet algae, and stirred at a speed of 300rpm for 12min , separate the algae cells and collect the extraction solvent containing biomass oil, re-add new extraction solvent, repeat the extraction operation steps, the wet algae body is extracted 3 times in total, and the total oil extraction rate is 50%; the collected biomass oil containing The extraction solvents were combined, vacuum rotary evaporation was carried out, the extraction solvent was recovered for reuse, and the obtained biomass oil was collected; the wet algae cells ...

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Abstract

The invention relates to the fields of biotechnology and energy, in particular to a method for extracting bio-oil by recycling botryococcus braunii cells, comprising the following steps of: (1) culturing and collecting wet fronds of botryococcus braunii; (2) putting the wet fronds into a stirring reactor, adding an extraction solvent composed of an organic solvent and an extraction assisting agent for extracting bio-oil, separating the frond cells and collecting the extraction solvent containing the bio-oil; and (3) recovering the extraction solvent from the extraction solvent containing the bio-oil collected in the step (2), and collecting the obtained bio-oil. According to the method provided by the invention, the wet fronds are directly used for extracting the bio-oil, thereby avoidingan algae powder drying step in the traditional microalgal oil extraction process, and saving the cost and energy consumption of microalgal oil extraction; and because the botryococcus braunii cells are repeatedly used, the oil accumulation speed is high, thereby shortening the time for culturing the botryococcus braunii, reducing the culturing cost, and improving the oil production intensity of the botryococcus braunii.

Description

technical field [0001] The invention relates to the fields of bioengineering technology and energy, in particular to a method for repeatedly utilizing staphylococcal algae cells to extract biomass oil. Background technique [0002] With the outbreak of the world oil crisis, our country is also faced with solving the problems of energy, environment and climate change. For the survival of human beings and the sustainable development of society, vigorously developing renewable energy is the only way. The significance of renewable energy lies not only in avoiding the impact of fossil energy depletion on my country's economy, but also in its ecological significance: reducing pollution, reducing the greenhouse effect, and improving the environment. Renewable and pollution-free biomass energy has become a research hotspot today. In biomass energy, high-yield oil-producing microalgae that can utilize various wastes have attracted widespread attention from relevant experts because of...

Claims

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Application Information

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IPC IPC(8): C12P7/64C11B1/10C10L1/02
CPCY02E50/10
Inventor 刘春朝徐玲王锋郭晨
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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