Sterilization composition containing tetraconazole and simeconazole
A technology of tetrafluconazole and its composition, which is applied in the field of pesticide compounding, can solve the problems such as the decline of disease control effect, and achieve the effects of high control effect, reduced agricultural cost, and low residual amount
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example 1
[0042] (1) Experimental object:
[0043] Cucumber powdery mildew pathogen was isolated and purified from cucumber powdery mildew-infected leaves in Shandong Shouguang cucumber greenhouse, cultured and preserved on PDA slant in 4°C refrigerator.
[0044] Test medium:
[0045] PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
[0046] AEA medium: yeast powder 5g / L, glycerol 20mL / L, MgSO 4 0.25g / L, NaNO 3 6g / L, KCl 0.5g / L, KH 2 PO 4 1.5g / L, agar powder 20g / L, deionized water 1L.
[0047] (2) Experimental medicine:
[0048] 94% Tefluconazole original drug, Italy SEG company, 95% Silfluzole original drug, Japan Sankyo Chemical Company.
[0049] Mix and match settings:
[0050] The percentages of 9% Tetraflumezol·Siliconazole are 1:8, 2:7, 3:6, 5:3, 7:2, that is, 1%+8%, 2%+7%, 3%+ 6%, 5%+3%, 7%+2% total 5 mixing combinations.
[0051] (3) Test method:
[0052] Toxicity determination of single agent against cucumber powdery mildew:
[0053] Tefluconazole and sil...
example 2
[0071] Biological Assay Example 2: Indoor Toxicity Determination of Tetrafluconazole and Sifluconazole Compounded to Wheat Powdery Mildew Pathogen.
[0072] Wheat powdery mildew pathogen is isolated and purified from wheat powdery mildew diseased leaves in the field, cultivated and stored in a 4°C refrigerator on a PDA slope.
[0073] Toxicity Assays for Single-Dose Tetrafluconazole and Silfluconazole, EC 50 The values were 2.19 mg / l and 5.74 mg / l, respectively.
[0074] Mix and match settings:
[0075] The percentages of 15% Tetraflumezol · Silfluzol are 2:13, 4:11, 6:9, 8:7, 10:5, that is, 2%+13%, 4%+11%, 6%+ 9%, 8%+7%, 10%+5% total 5 mixing combinations.
[0076] Test method is the same as biological assay embodiment 1
[0077] Experimental results and analysis:
[0078] Table 3 Toxicity test of tetrafluconazole and silfluazole compound against wheat powdery mildew
[0079]
[0080] Table 4 Combined toxicity test of tetrafluconazole and silfluazole compound again...
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