The invention relates to a weedicide composition containing Tembotrione, Terbuthylazine and Isoxadifen-ethyl. The weight part ratio of the three active components in the preparation is (1-10):(1-50):(0.5-10), and the total weight part of the active components accounts for 2.5-70% of the total weight part of the whole preparation. The weedicide composition can be prepared into multiple agricultural chemical preparation forms such as wettable powder, suspending agents, dispersible suspending agents, microemulsion, emulsion in water, granules and water-dispersible granules, and is used for controlling multiple grassy weeds, broadleaf weeds and sedge weeds. The weedicide composition can control the multiple grassy weeds, broadleaf weeds and sedge weeds capable of being controlled by single use of the Tembotrione, improves the control effect on part of the grassy weeds and broadleaf weeds, widens the weed control spectrum, additionally has a particular sealing effect of the Terbuthylazine, improves the degree of safety for corns, lowers the dosage, saves the control cost, improves the safety for succeeding crops, and reduces pollution to the environment.