Novel environment-friendly germicidal composition
A composition and compound technology, applied in the direction of fungicides, biocides, biocides, etc., to achieve the effects of reducing agricultural costs, reducing the amount of pesticides used, and generally preserving freshness and increasing production
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example 1
[0045] (1) Experimental object:
[0046] Cucumber downy mildew pathogen was isolated and purified from cucumber downy mildew diseased leaves in Shouguang cucumber greenhouse in Shandong Province, and cultured and preserved on PDA slopes in a 4°C refrigerator.
[0047] Test medium:
[0048] PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
[0049] AEA medium: yeast powder 5g / L, glycerol 20mL / L, MgSO 4 0.25g / L, NaNO 3 6g / L, KCl 0.5g / L, KH 2 PO 4 1.5g / L, agar powder 20g / L, deionized water 1L.
[0050] (2) Experimental medicine:
[0051] 90% Cyflufenamide technical product, Germany Bayer Crop Science Co., Ltd., 93.5% Cyazofamid technical product, Japan Ishihara Sangyo Co., Ltd.
[0052] Mix and match settings:
[0053] The content percentages of 17% Cyflufenad Cyazofamid are 3:14, 5:12, 7:10, 9:8, 11:6, namely 3%+14%, 5%+12%, 7%+ 10%, 9%+8%, 11%+6% total 5 mixing combinations.
[0054] (3) Test method:
[0055] Toxicity determination of single agent agai...
example 2
[0073] Biological Assay Example 2: Indoor Toxicity Test of Cyflufen and Prochloraz on Cucumber Anthracnose Pathogen.
[0074] 97% prochloraz technical, Nantong Jiangshan Pesticide Chemical Co., Ltd., Jiangsu Province.
[0075] Cucumber anthracnose pathogen was isolated and purified from cucumber anthracnose-infected leaves in Shouguang cucumber shed, and cultured and preserved on PDA slant at 4°C refrigerator.
[0076] Toxicity assays for single doses of cycloflufen and prochloraz, EC 50 The values were 3.84 mg / l and 8.57 mg / l, respectively.
[0077] Mix and match settings:
[0078] The content percentages of 20% cycloflufen and prochloraz are 3:17, 5:15, 7:13, 9:11, 11:9, namely 3%+17%, 5%+15%, 7%+ 13%, 9% + 11%, 11% + 9%, a total of 5 mixed combinations.
[0079] Test method is the same as biological assay embodiment 1
[0080] Experimental results and analysis:
[0081] Table 3 Toxicity test of the combination of cycloflufen and prochloraz against the pathogen of cu...
example 3
[0086] Biological Assay Example 3: Indoor Toxicity Test of Cyflufen and Prochloraz on Citrus Green Mold.
[0087] The citrus green mold fungus was isolated and purified from the diseased citrus in the citrus orchard in Jiangxi, cultured and preserved on a PDA slant in a refrigerator at 4°C.
[0088] Toxicity assays for single doses of cycloflufen and prochloraz, EC 50 The values were 4.13 mg / l and 8.29 mg / l, respectively.
[0089] Mix and match settings:
[0090] The content percentages of 18% Cyflufenamide Thiabendazole are 3:15, 5:13, 7:11, 9:9, 11:7, namely 3%+15%, 5%+13%, 7% +11%, 9%+9%, 11%+7%, a total of 5 mixed combinations.
[0091] Test method is similar to biological assay embodiment 1
[0092] results and analysis:
[0093] Table 5 Combined toxicity of cycloflufen and prochloraz against citrus green mold
[0094]
[0095] Table 6 Combined toxicity of cycloflufen and prochloraz against citrus green mold
[0096] Test drug
mass ratio
Measu...
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