Antibacterial composition containing tetraconazole and pyraclostrobin
A technology of tetrafluoroconazole and pyraclostrobin, applied in the directions of fungicides, biocides, biocides, etc., can solve the problems of pyraclostrobin's quick-acting effect and poor stability, and achieves the reduction of pesticide usage, The effect of stable effect and reduction of agricultural cost
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example 1
[0041] (1) Experimental object:
[0042]Cucumber powdery mildew pathogen was isolated and purified from cucumber powdery mildew-infected leaves in Shandong Shouguang cucumber greenhouse, cultured and preserved on PDA slant in 4°C refrigerator.
[0043] Test medium:
[0044] PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
[0045] AEA medium: yeast powder 5g / L, glycerol 20mL / L, MgSO 4 0.25g / L, NaNO 3 6g / L, KCl 0.5g / L, KH 2 PO 4 1.5g / L, agar powder 20g / L, deionized water 1L.
[0046] (2) Experimental medicine:
[0047] 94% Tefluconazole technical, Italy SEG company, 95% pyraclostrobin technical, BASF Europe.
[0048] Mix and match settings:
[0049] The content percentages of 14% tetraflumezole pyraclostrobin are 1:13, 3:11, 5:9, 7:7, 9:5, that is, 1%+13%, 3%+11%, 5% +9%, 7%+7%, 9%+5%, a total of 5 mixing combinations.
[0050] (3) Test method:
[0051] Toxicity determination of single agent against cucumber powdery mildew:
[0052] Tefluconazole and py...
example 2
[0070] Biological Assay Example 2: Indoor Toxicity Test of Tefluconazole and Pyraclostrobin Compound on Cucumber Downy Mildew.
[0071] Cucumber downy mildew, isolated and purified from the diseased leaves of cucumbers in the field, was cultured and preserved on a PDA slope at 4°C in a refrigerator.
[0072] Toxicity assays for single doses of tetrafluconazole and pyraclostrobin, EC 50 The values were 4.13 mg / l and 7.29 mg / l, respectively.
[0073] Mix and match settings:
[0074] The content percentages of 15% tetraflumezole pyraclostrobin are 2:13, 4:11, 6:9, 8:7, 10:5, namely 2%+13%, 4%+11%, 6% +9%, 8%+7%, 10%+5%, a total of 5 mixing combinations.
[0075] Test method is the same as biological assay embodiment 1
[0076] Experimental results and analysis:
[0077] Table 3 Toxicity test of tetrafluconazole and pyraclostrobin compound against cucumber downy mildew
[0078]
[0079]
[0080] Table 4 Combined toxicity test of tetrafluconazole and pyraclostrobin co...
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