Germicide composition in combination with bupirimate
A composition and fungicide technology, applied in the direction of fungicides, biocides, biocides, etc., to achieve the effect of less usage, long-lasting drug effect, and reduced usage
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example 1
[0051] (1) Experimental object:
[0052] Cucumber powdery mildew pathogen was isolated and purified from cucumber powdery mildew-infected leaves in Shandong Shouguang cucumber greenhouse, cultured and preserved on PDA slant in 4°C refrigerator.
[0053] Test medium:
[0054] PDA medium: potato 200g, glucose 20g, agar 20g, water 1000ml.
[0055] AEA medium: yeast powder 5g / L, glycerol 20mL / L, MgSO 4 0.25g / L, NaNO 3 6g / L, KCL0.5g / L, KH 2 PO 4 1.5g / L, agar powder 20g / L, deionized water 1L.
[0056] (2) Experimental medicine:
[0057] 95% pyrimethrin sulfonate technical product, provided by Qingdao Hailier R&D Center Synthesis Institute, 95% pyraclostrobin technical product, BASF Europe.
[0058] Mix and match settings:
[0059] The content percentages of 18% pyrifluzate and pyraclostrobin are 3:15, 5:13, 7:11, 9:9, 11:7, that is, 3%+15%, 5%+13%, 7%+11%, 9%+9%, 11%+7% total 5 mixing combinations.
[0060] (3) Test method:
[0061] Toxicity determination of single agent ...
example 2
[0079] Biological Assay Example 2: Indoor Toxicity Determination of Pyrifluzate and Azoxystrobin Compound to Cucumber Downy Mildew.
[0080] 98% azoxystrobin technical product, Shangyu Yingtai Fine Chemical Co., Ltd.
[0081] Cucumber downy mildew pathogen was isolated and purified from cucumber downy mildew diseased leaves in Shouguang cucumber greenhouse in Shandong Province, and cultured and preserved on PDA slopes in a 4°C refrigerator.
[0082] Toxicity Assay for Single Doses of Pyriflux and Azoxystrobin, EC 50 The values were 5.11 mg / l and 7.23 mg / l, respectively.
[0083] Mix and match settings:
[0084] The content percentages of 21% pyrifluzate and azoxystrobin are 4:17, 6:15, 8:13, 10:11, 12:9, namely 4%+17%, 6%+15%, 8% %+13%, 10%+11%, 12%+9%, a total of 5 mixing combinations.
[0085] Test method is similar to biological assay embodiment 1
[0086] Experimental results and analysis:
[0087] Table 3 Toxicity test of pyriflux and azoxystrobin compound against...
example 3
[0092] Biological Assay Example 3: Indoor Toxicity Determination of Pyrimethol Sulfonate and Phenostrobin Compound to Cucumber Leaf Spot.
[0093] 98% dikestrobin technical, Zhejiang Hetian Chemical Co., Ltd.
[0094] Cucumber leaf spot pathogen was isolated and purified from cucumber leaf spot diseased leaves in Shouguang cucumber greenhouse in Shandong Province, and cultured and preserved on PDA slant in 4°C refrigerator.
[0095] Toxicity Assay for Single Doses of Pyrimetholsulfonate and Dikestrobin, EC 50 The values were 4.86 mg / l and 7.55 mg / l, respectively.
[0096] Mix and match settings:
[0097] The content percentages of 16% pyrifluzate and dikestrobin are 3:13, 5:11, 7:9, 9:7, 11:5, namely 3%+13%, 5%+11% , 7%+9%, 9%+7%, 11%+5%, a total of 5 mixing combinations.
[0098] Test method is similar to biological assay embodiment 1
[0099] results and analysis:
[0100] Table 5 Combined virulence of pyrithrimol sulfonate and dikestrobin against Cucumber leaf spot ...
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