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Acinetobacter baumannii capable of efficiently degrading imazamox
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A technology of Acinetobacter baumannii and imazethapyr, which is applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., and can solve problems such as long residual time, screening of degrading strains, less application, and influence on the normal growth of subsequent crops. , to achieve the effect of high-efficiency degradation
Active Publication Date: 2012-12-19
海林市中农国泰生物科技有限公司
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Because this product has a longer duration than other herbicides, it can control weeds for 4 to 6 months, so it has been widely used in North America, Europe, Southeast Asia and other countries in the early 1990s. A herbicide that is used in large quantities at home and abroad, but this type of herbicide remains in the soil for a long time, and it is easy to affect the normal growth of subsequent crops
[0003] At present, the research reports on imazethapyr at home and abroad mainly focus on the use and injury, but less about the screening and application of degrading strains
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specific Embodiment approach 1
[0012] Specific embodiment 1: In this embodiment, the Acinetobacter baumannii that efficiently degrades the imazamox strain is Acinetobacter baumannii IB5 (Acinetobacter baumannii IB5), which is deposited in the General Microbiology Center (CGMCC) of the China Microbial Culture Collection Management Committee (CGMCC) The preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The preservation date is April 1, 2011, and the preservation number is CGMCC No. 4728.
[0013] In this embodiment, Acinetobacter baumannii IB5 (Acinetobacter baumannii IB5) is a gram-negative bacterium, rod-shaped, with a cell size of (0.3μm~0.5μm)×(0.8μm~2.2μm), without flagella, and produces a bacterial membrane. Cultivated on inorganic salt solid medium, the colonies are round and milky white in color.
[0014] Physiological and biochemical identification of Acinetobacter baumannii IB5 (Acinetobacter baumannii IB5) with reference to the eighth edition of Berger Bacterial Identi...
specific Embodiment approach 2
[0017] Specific implementation manner 2: In this implementation manner, Acinetobacter baumannii IB5 (Acinetobacter baumannii IB5) was selected from soil contaminated by herbicides in Nehe City, Heilongjiang Province, China. The screening is carried out according to the following steps: Take 5g of the farmland soil that has been long-term applied methazine phytotoxicity in Nehe City, Heilongjiang Province, put it in a sterilized jar, add 50mL of normal saline, shake for 30s, filter with 8 layers of gauze, and take the filtrate 1mL was added to the inorganic salt medium and cultured at 30°C and 180r / min for 7-10 days, during which time it was continuously supplemented with imazamo to increase the concentration of imazamo to 800mg / L to obtain enrichment Culture solution; The inorganic salt solid medium (1000mL inorganic salt solid medium is made of 1.0g NaCl, 1.0g NH 4 NO 3 , 1.5g of K 2 HPO 4 , 0.5g of KH 2 PO 4 , 0.1g of MgSO 4 ·7H 2 O, 20g of agar and the remainder of distilled ...
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Abstract
The invention dislcoses acinetobacter baumannii capable of efficiently degrading imazamox, which relates to acinetobacter baumannii. The invention provides an acinetobacter baumannii strain, which is capable of efficiently degrading imazamox and provides theoretical and microbial resource foundations for the environmental modification and degradation mechanism research of an imazamox weedicide. The acinetobacter baumannii capable of efficiently degrading imazamox, disclosed by the invention, is acinetobacter baumannii IB5 which is collected in the China General Microbiological Culture Collection Center (Address: No.3, Yard 1, Beichen Road West, Chaoyang District, Beijing) with the collection number CGMCC No.4728 on April 1, 2011. The acinetobacter baumannii IB5 is capable of efficiently degrading imazamox.
Description
Technical field [0001] The present invention relates to a strain of Acinetobacter baumannii. Background technique [0002] Imazamox (Imazamox) is an imidazolinone special herbicide for soybean fields developed by BASF in Germany (formerly American Cyanamide Company) in the early 1980s. Grass weeds and a variety of shrubs and deciduous trees are active. Since the product has a longer duration than other biocidal herbicides and can control weeds for 4-6 months, it has been widely used in North America, Europe, Southeast Asia and other countries in the early 1990s. A type of herbicide used in large quantities at home and abroad, but this kind of herbicide stays in the soil for a long time, which easily affects the normal growth of subsequent crops. [0003] At present, the domestic and foreign research reports on imazamox mainly focus on the usage and phytotoxicity, while the selection and application of degrading strains are few. Summary of the invention [0004] The invention prov...
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